[Detection of a steady increase in endonuclease activity in dog liver cell nuclei after long term arterial hypotension and in post-resuscitation period].

Presence of Ca2+, Mg(2+)-dependent endonuclease activity was found in dog liver cell nuclei. Specific characteristics of chromatin autolysis were also studied in liver cell nuclei after 2-4 hrs long arterial hypotension as compared with that within the later restoration period 1-3 months. The rate of DNA acid-soluble fraction accumulation correlated directly with the arterial hypotension duration. Quantitative evaluation of the liver tissue Ca2+, Mg(2+)-dependent endonuclease activity was undertaken under conditions of hemorrhagic shock. The enzymatic activity was not normalized both after death and in the postresuscitation period. Analysis of the chromatin autolysis and of alterations in the enzymatic activity during postresuscitation period enabled to suggest that the isozyme spectrum of Ca2+, Mg(2+)-dependent endonuclease was altered.