模拟胃肠道消化条件下亚麻籽（Linum usitatissimum L.）蛋白中血管紧张素转化酶抑制肽的释放。

Release of angiotensin I-converting enzyme inhibitory peptides from flaxseed (Linum usitatissimum L.) protein under simulated gastrointestinal digestion.

机构信息

Department of Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon SK, Canada S7N 5A8.

出版信息

J Agric Food Chem. 2011 Sep 14;59(17):9596-604. doi: 10.1021/jf202000e. Epub 2011 Aug 8.

DOI:10.1021/jf202000e

Abstract

The scope of this study was to determine the ability of flaxseed (Linum usitatissimum L.) proteins to release angiotensin I-converting enzyme inhibitory (ACEI) peptides during simulated gastrointestinal (GI) digestion using a static (SM; no absorption in the intestinal phase) and a dynamic model (DM; simultaneous absorption of digested products in the intestinal phase via passive diffusion). Gastric and gastric + small intestinal digests of flaxseed proteins of both models possessed ACEI activity. The ACEI activity of the gastric + small intestinal digest in the DM (IC(50) unabsorbed, 0.05 mg N/mL; IC(50) absorbed, 0.04 mg N/mL) was significantly higher (p < 0.05) than that of the SM (IC(50), 0.39 mg N/mL). Two peptides, a pentapeptide (Trp-Asn-Ile/Leu-Asn-Ala) and a hexapeptide (Asn-Ile/Leu-Asp-Thr-Asp-Ile/Leu), were identified in the most active ACEI fraction (0.5-1 kDa) of the absorbable flaxseed protein digest by de novo sequencing.

摘要

本研究旨在利用静态（SM；肠相无吸收）和动态模型（DM；肠相同时通过被动扩散吸收消化产物），确定亚麻籽（Linum usitatissimum L.）蛋白在模拟胃肠道（GI）消化过程中释放血管紧张素 I 转化酶抑制（ACEI）肽的能力。两种模型的亚麻籽蛋白胃和胃+小肠消化物均具有 ACEI 活性。DM 中胃+小肠消化物的 ACEI 活性（未吸收的 IC(50)，0.05mg N/mL；吸收的 IC(50)，0.04mg N/mL）明显高于 SM（IC(50)，0.39mg N/mL）（p<0.05）。通过从头测序，在可吸收的亚麻籽蛋白消化物中最具活性的 ACEI 级分（0.5-1kDa）中鉴定出两种肽，五肽（Trp-Asn-Ile/Leu-Asn-Ala）和六肽（Asn-Ile/Leu-Asp-Thr-Asp-Ile/Leu）。