Tiengo A, Faria M, Netto F M
Dept. of Food and Nutrition, Univ. of Campinas-Unicamp, Brazil.
J Food Sci. 2009 Jun;74(5):H121-6. doi: 10.1111/j.1750-3841.2009.01145.x.
Amaranth seeds have been considered as an excellent alternative or complementary source of food protein due to their balanced amino acid composition. However, their potential as a source of bioactive peptides has not been explored. The present study is aimed at characterizing and evaluating the activity of the angiotensin converting enzyme inhibitor of the amaranth protein concentrate and of hydrolysates produced with Alcalase. The protein concentrate, after simulated gastrointestinal digestion, showed lower angiotensin converting enzyme-inhibitory activity (IC(50) of 0.439 +/- 0.018 mg protein/mL and 0.475 +/- 0.021 mg protein/mL, for untreated and heat treated protein concentrate, respectively) than the hydrolysates produced with Alcalase, before and after simulated gastrointestinal digestion (IC(50) 0.118 +/- 0.009, 0.123 +/- 0.007, 0.137 +/- 0.002, and 0.176 +/- 0.014 mg protein/mL, respectively). The simulated gastrointestinal digestion (pepsin-pancreatin) did not significantly alter the angiotensin-converting enzyme inhibiting activity of the Alcalase hydrolysates, suggesting that the peptides of the hydrolysates were resistant to gastrointestinal hydrolysis. These results highlight the angiotensin converting enzyme-inhibitory potential of amaranth proteins, which is an indication of their health-promoting potential.
由于苋菜籽的氨基酸组成平衡，它们被认为是食物蛋白质的优质替代或补充来源。然而，它们作为生物活性肽来源的潜力尚未得到探索。本研究旨在表征和评估苋菜籽浓缩蛋白以及用碱性蛋白酶生产的水解产物的血管紧张素转换酶抑制剂活性。模拟胃肠道消化后的浓缩蛋白，与用碱性蛋白酶生产的水解产物在模拟胃肠道消化前后相比，显示出较低的血管紧张素转换酶抑制活性（未处理和热处理的浓缩蛋白的IC50分别为0.439±0.018 mg蛋白/mL和0.475±0.021 mg蛋白/mL，而水解产物的IC50分别为0.118±0.009、0.123±0.007、0.137±0.002和0.176±0.014 mg蛋白/mL）。模拟胃肠道消化（胃蛋白酶-胰蛋白酶）并未显著改变碱性蛋白酶水解产物的血管紧张素转换酶抑制活性，这表明水解产物中的肽对胃肠道水解具有抗性。这些结果突出了苋菜籽蛋白的血管紧张素转换酶抑制潜力，这表明它们具有促进健康的潜力。
