大脑中天然GABAA受体中γ2亚基蛋白的鉴定。
Identification of the gamma 2-subunit protein in native GABAA receptors in brain.
作者信息
Benke D, Mertens S, Trezciak A, Gillessen D, Mohler H
机构信息
Institute of Pharmacology, University of Zurich, Switzerland.
出版信息
Eur J Pharmacol. 1990 Oct 30;189(4-5):337-40. doi: 10.1016/0922-4106(90)90127-j.
GABAA receptors with functional benzodiazepine receptors have been reconstituted by coexpression of alpha-, beta- and gamma-subunit cDNAs. In brain, proteins of the alpha- and beta-subunits, but not the gamma 2-subunit have been identified. Using an antipeptide antiserum, we now demonstrate that the gamma 2-subunit is a protein of 43 kDa. It is present in at least 50% of GABAA receptors, as shown by immunoprecipitation.
通过共表达α、β和γ亚基的互补DNA，已成功重构出具有功能性苯二氮䓬受体的GABAA受体。在大脑中，已鉴定出α和β亚基的蛋白质，但未鉴定出γ2亚基的蛋白质。利用抗肽抗血清，我们现在证明γ2亚基是一种43 kDa的蛋白质。免疫沉淀结果显示，它存在于至少50%的GABAA受体中。
Zotero 插件