大鼠器官中果糖1,6-二磷酸酶同工酶的调查及该酶在大鼠胎儿中的个体发育表达

Survey of fructose 1,6-bisphosphatase isoenzyme in rat organs and ontogenic expression of the enzyme in rat fetus.

Mizunuma H, Tashima Y

Department of Biochemistry, Akita University School of Medicine, Japan.

Int J Biochem. 1990;22(8):883-7. doi: 10.1016/0020-711x(90)90292-b.

Abstract

Among eleven tissues of rat, the liver type of fructose 1,6-bisphosphatase (FBPase) subunit was detected in the liver, kidney, testis, pancreas and lung by Western blot analysis using anti-(liver FBPase) or anti-(muscle FBPase) serum. 2. The muscle type of the enzyme subunit was detected only in the pancreas other than skeletal muscle. Both types of the enzyme subunit were found in the pancreas. 3. Neither anti-(liver FBPase) nor anti-(muscle FBPase) serum detected the band of enzyme subunit on the blots of the extracts of brain, heart, small intestinal mucosa, spleen and placenta. 4. FBPase is present in fetal rat liver at least as early as the 14th day of gestation. 5. In agreement with the increase in immunological staining density, the level of the enzyme activity in fetal liver increased exponentially during fetal development. 6. The muscle enzyme was not detected until the fetus reached the 19th day of gestation.

摘要

在大鼠的11种组织中，使用抗（肝脏果糖-1,6-二磷酸酶）或抗（肌肉果糖-1,6-二磷酸酶）血清通过蛋白质免疫印迹分析在肝脏、肾脏、睾丸、胰腺和肺中检测到了肝脏型果糖-1,6-二磷酸酶（FBPase）亚基。2. 该酶亚基的肌肉型仅在胰腺中而非骨骼肌中检测到。在胰腺中发现了两种类型的酶亚基。3. 抗（肝脏FBPase）血清和抗（肌肉FBPase）血清均未在脑、心脏、小肠黏膜、脾脏和胎盘提取物的印迹上检测到酶亚基条带。4. 果糖-1,6-二磷酸酶至少在妊娠第14天就存在于胎鼠肝脏中。5. 与免疫染色密度的增加一致，胎肝中的酶活性水平在胎儿发育过程中呈指数增加。6. 直到胎儿达到妊娠第19天才检测到肌肉酶。