Division of Mass Spectrometry Research, Korea Basic Science Institute, Cheongwon, Chungbuk 363-883, Republic of Korea.
Clin Chim Acta. 2011 Oct 9;412(21-22):1978-82. doi: 10.1016/j.cca.2011.07.008. Epub 2011 Jul 18.
We evaluated whether direct tissue matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) analysis of lipids may distinguish intrahepatic cholangiocarcinomas from adjacent normal tissue and from other adenocarcinomas that frequently metastasize to liver.
Four pairs of frozen surgical specimens of cholangiocarcinomas and adjacent normal tissue were analyzed using histology-directed, MALDI MS analysis. 2,5-dihydroxybenzoic acid / α-cyano-4-hydroxycinnamic acid were manually deposited on tumor-rich areas, and mass spectra were acquired using a MALDI-time of flight instrument.
Cholangiocarcinomas and adjacent normal tissue samples demonstrated different lipid profiles, as evidenced by permutation P value<0.05 for the cross-validated misclassification rate. Cancer-associated lipid alteration was similar between cholangiocarcinomas and pancreatic cancers, but not between cholangiocarcinomas and colorectal cancers. Baseline lipid profiles were different between cholangiocarcinoma and colorectal cancers.
MALDI MS analysis of lipid distinguishes cancerous epithelium of cholangiocarcinoma from adjacent normal tissue, and between cholangiocarcinomas and colorectal cancers.
我们评估了直接组织基质辅助激光解吸/电离（MALDI）质谱（MS）分析脂质是否可以区分肝内胆管癌与邻近的正常组织，以及经常转移到肝脏的其他腺癌。
使用组织学定向 MALDI MS 分析对四对肝内胆管癌和相邻正常组织的冷冻手术标本进行分析。将 2,5-二羟基苯甲酸/α-氰基-4-羟基肉桂酸手动沉积在富含肿瘤的区域，并使用 MALDI-飞行时间仪器获取质谱。
肝内胆管癌和相邻正常组织样本表现出不同的脂质谱，这一点通过交叉验证错误分类率的置换 P 值<0.05 得到证明。胆管癌和胰腺癌之间的癌症相关脂质改变相似，但胆管癌和结直肠癌之间则不同。胆管癌和结直肠癌之间的基线脂质谱也不同。
MALDI MS 分析脂质可区分胆管癌的癌上皮与邻近的正常组织，以及胆管癌与结直肠癌。
