Bélanger B, Caron S, Bélanger A, Dupont A
Laboratory of Molecular Endocrinology, CHUL Research Center, Quebec, Canada.
J Endocrinol. 1990 Dec;127(3):505-11. doi: 10.1677/joe.0.1270505.
The presence and production of 5-ene-steroid fatty acid esters (SFA) has been previously reported in bovine adrenals. A study was conducted, using a series of chromatographic procedures and radioimmunoassays, to determine the levels of SFA in adrenals from man, cattle, dog, rat and guinea-pig, and to assess, in both rats and guinea-pigs, the effect of ACTH on SFA production by adrenals and their subsequent secretion into the circulation. The effects of ACTH on plasma SFA and non-conjugated steroid levels were also investigated in human subjects. Our data indicated that adrenal pregnenolone fatty acid ester (PREG-FA) levels were below 40% of PREG levels in cattle, dog, rat and guinea-pig while, in man, PREG-FA levels were threefold those of PREG. A large proportion of dehydroepiandrosterone (DHEA) and 5-androstene-3 beta, 17 beta-diol were present as fatty acid ester derivatives in the adrenals of all species, with the exception of cattle. In both rats and guinea-pigs, administration of ACTH caused a sharp increase in adrenal PREG of approximately threefold which lasted for 6 h, while the concentration of adrenal PREG-FA was slightly increased for a short time. In plasma, however, a marked rise in PREG-FA occurred, while the changes in PREG levels were much lower than those of its acylated counterpart. In man, PREG and DHEA concentrations were rapidly stimulated two-fold in the first 30 min following the administration of ACTH, while PREG-FA and DHEA-FA levels were increased by approximately 2.5-fold (P less than 0.01) at 120 and 180 min.(ABSTRACT TRUNCATED AT 250 WORDS)
先前已有报道称牛肾上腺中存在并能产生5-烯-甾体脂肪酸酯（SFA）。本研究采用一系列色谱分析方法和放射免疫分析法，测定人、牛、犬、大鼠和豚鼠肾上腺中SFA的水平，并评估促肾上腺皮质激素（ACTH）对大鼠和豚鼠肾上腺SFA产生及其随后分泌入循环系统的影响。同时还研究了ACTH对人体血浆SFA和非共轭甾体水平的影响。我们的数据表明，牛、犬、大鼠和豚鼠肾上腺中孕烯醇酮脂肪酸酯（PREG-FA）的水平低于孕烯醇酮（PREG）水平的40%，而在人体中，PREG-FA的水平是PREG的三倍。除牛以外，所有物种的肾上腺中，很大一部分脱氢表雄酮（DHEA）和5-雄烯-3β,17β-二醇都以脂肪酸酯衍生物的形式存在。在大鼠和豚鼠中，注射ACTH后，肾上腺PREG急剧增加约三倍，持续6小时，而肾上腺PREG-FA的浓度在短时间内略有增加。然而，在血浆中，PREG-FA显著升高，而PREG水平的变化远低于其酰化对应物。在人体中，注射ACTH后的前30分钟内，PREG和DHEA浓度迅速增加两倍，而PREG-FA和DHEA-FA水平在120分钟和180分钟时增加约2.5倍（P<0.01）。（摘要截短于250字）
