葡萄柚汁对人淋巴细胞中过氧化氢诱导的遗传毒性的抑制作用。
Inhibitory effect of grapefruit juice on the genotoxicity induced by hydrogen peroxide in human lymphocytes.
机构信息
Instituto Nacional de Perinatología, SSA, Montes Urales 800, Lomas Virreyes, 11000 México DF, Mexico.
出版信息
Food Chem Toxicol. 2011 Nov;49(11):2947-53. doi: 10.1016/j.fct.2011.07.003. Epub 2011 Jul 12.
By means of the comet assay we demonstrated a strong effect by hydrogen peroxide (HP) and no damage by grapefruit juice (GJ) in human lymphocytes. Cells exposed to HP and treated with three concentrations of GJ (10-90 min) showed an increase of DNA damage by HP over the control level, and a decrease of such damage by GJ. With the comet assay plus formamidopyrimidine-DNA-glycosylase we found the strongest increase of DNA damage by HP over the control level, and the strongest reduction of such damage by GJ. By applying the comet/FISH method we determined 98% of the p53 gene signals in the comet head of control cells along the experiment (10-90 min), in contrast with about 90% signals in the comet tail of cells exposed to HP. Cells treated with both agents showed a significant, concentration/time dependent return of p53 signals to the head, suggesting enhancement of the gene repair. Finally, with the annexin V assay we found an increase in apoptosis and necrosis by HP, and no effect by GJ; when GJ was added to HP treated cells no modification was observed in regard to apoptosis, although a decrease of necrosis was observed.
通过彗星试验，我们证明了过氧化氢（HP）有很强的作用，而葡萄柚汁（GJ）对人类淋巴细胞没有损伤。将细胞暴露于 HP 并以三种浓度的 GJ（10-90 分钟）处理，结果显示 HP 引起的 DNA 损伤比对照水平增加，而 GJ 则减少了这种损伤。通过彗星试验加氨嘧啶-DNA-糖苷酶处理，我们发现 HP 引起的 DNA 损伤比对照水平增加了最强，而 GJ 对这种损伤的减少最强。通过应用彗星/FISH 方法，我们确定了在实验过程中（10-90 分钟），对照细胞的彗星头部有 98%的 p53 基因信号，而暴露于 HP 的细胞的彗星尾部只有约 90%的信号。用两种药物处理的细胞显示出 p53 信号显著、浓度/时间依赖性地回到头部，提示基因修复增强。最后，通过 Annexin V 试验，我们发现 HP 导致细胞凋亡和坏死增加，而 GJ 没有影响；当将 GJ 添加到 HP 处理的细胞中时，凋亡没有观察到变化，尽管坏死减少。
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