Department of Pathology, Graduate School of Medicine, Kyung Hee University, College of Medicine, Seoul 134-727, Republic of Korea.
Hum Pathol. 2012 Jan;43(1):62-8. doi: 10.1016/j.humpath.2011.04.007. Epub 2011 Jul 20.
Beclin-1 is a Bcl-2-interacting protein, and it may delay cell cycle progression and induce autophagy. The function and expression of Beclin-1 and Bcl-2 in squamous cell carcinoma and adenocarcinoma of the lung remain largely unknown. Herein, we investigated the expression of Beclin-1 and Bcl-2 in squamous cell carcinoma and adenocarcinoma of the lung. Tissue samples from 262 cases were used in this study. Immunohistochemical staining for Beclin-1 and Bcl-2 were conducted using a tissue microarray. In squamous cell carcinoma, Beclin-1 expression was strongly positive in 48 (28.6%) of 168 samples, it was moderately positive in 42 (25.0%) of 168 samples, and it was negative or weakly positive in 78 (46.4%) of 168 samples. In adenocarcinoma, Beclin-1 expression was strongly positive in 26 (27.7%) of 94 samples, it was moderately positive in 27 (28.7%) of 94 samples, and it was negative or weakly positive in 41 (43.6%) of 94 samples. Beclin-1 expression was inversely correlated with the tumor size and the primary tumor stage (pT) in both types of tumor. Especially, the TNM stage of adenocarcinoma was inversely correlated with Beclin-1 expression. Our results suggest that a progressively reduced Beclin-1 expression is correlated with the primary tumor growth of squamous cell carcinoma and adenocarcinoma of the lung.
Beclin-1 是一种与 Bcl-2 相互作用的蛋白，它可能会延迟细胞周期的进展并诱导自噬。Beclin-1 和 Bcl-2 在肺鳞癌和腺癌中的功能和表达仍知之甚少。在此，我们研究了 Beclin-1 和 Bcl-2 在肺鳞癌和腺癌中的表达。本研究使用了 262 例组织样本。使用组织微阵列进行了 Beclin-1 和 Bcl-2 的免疫组织化学染色。在肺鳞癌中，168 例样本中有 48 例（28.6%）Beclin-1 表达强阳性，有 42 例（25.0%）Beclin-1 中度阳性，有 78 例（46.4%）Beclin-1 表达阴性或弱阳性。在肺腺癌中，94 例样本中有 26 例（27.7%）Beclin-1 表达强阳性，有 27 例（28.7%）Beclin-1 中度阳性，有 41 例（43.6%）Beclin-1 表达阴性或弱阳性。Beclin-1 的表达与两种类型肿瘤的肿瘤大小和原发性肿瘤分期（pT）呈负相关。特别是，腺癌的 TNM 分期与 Beclin-1 的表达呈负相关。我们的结果表明，Beclin-1 表达的逐渐降低与肺鳞癌和腺癌的原发性肿瘤生长有关。
