Department of Clinical Chemistry, New Cross Hospital, Wolverhampton, West Midlands, UK.
J Clin Pathol. 2011 Nov;64(11):1019-20. doi: 10.1136/jclinpath-2011-200206. Epub 2011 Jul 21.
To investigate whether incorrect order of draw of blood samples during phlebotomy causes in vitro potassium ethylenediaminetetraacetic acid EDTA (kEDTA) contamination of blood samples.
Serum kEDTA, potassium, calcium, magnesium, alkaline phosphatase, zinc and iron concentrations were measured in blood samples drawn before and after collecting blood into kEDTA containing sample tubes by an experienced phlebotomist using the Sarstedt Safety Monovette system.
EDTA was undetectable in all samples. The concentrations of other analytes were similar in blood samples drawn before and after collection of the EDTA blood sample.
Order of draw of blood samples using the Sarstedt Safety Monovette system has no effect on serum biochemistry results, when samples are taken by an experienced phlebotomist.
调查在采集血样时，如果采血顺序不正确，是否会导致血液样本中体外乙二胺四乙酸钾（kEDTA）污染。
采用 Sarstedt Safety Monovette 系统，由经验丰富的采血护士采集血样前和采集含 kEDTA 的样本管后，分别检测血清 kEDTA、钾、钙、镁、碱性磷酸酶、锌和铁浓度。
所有样本中均未检测到 EDTA。在采集 EDTA 血样前后采集的血液样本中，其他分析物的浓度相似。
当由经验丰富的采血护士采血时，使用 Sarstedt Safety Monovette 系统按顺序采集血样不会影响血清生化结果。
