General Dentistry Program, Faculty of Dentistry at San Luis Potosi University, Av. Dr. Manuel Nava # 2, Zona Universitaria, San Luis Potosi, SLP, México
Dent Mater J. 2011;30(4):546-53. doi: 10.4012/dmj.2011-023. Epub 2011 Jul 21.
The aim was to characterize by atomic force microscopy (AFM) the nanostructure of human dentin surfaces affected by dental fluorosis (DF) before and after phosphoric acid etching. This study included 240 human dentin samples classified according to the severity of DF, which were divided into four groups using the Thylstrup-Fejerskov Index (TFI). Samples were analyzed by AFM before and after acid etching for 15, 30, and 60 s. The roughness (R(a)) for healthy dentin, and dentin with mild, moderate, and severe fluorosis were 440 nm, 442 nm, 445 nm, and 449 nm, respectively. After 15, 30, and 60 s of acid etching, all healthy and fluorotic dentin samples increased in roughness (p<0.05). The diameter of dentinal tubule orifices (D(t)) in healthy human dentin increased after acid etching for 60 s. We conclude that effective etching times are 15 s for healthy and mild dentin fluorosis, 30-s for moderately fluorosed dentin, and 45-60 s for severe fluorotic dentin.
目的是通过原子力显微镜（AFM）来描述氟斑牙（DF）对人牙本质表面纳米结构的影响，包括在磷酸酸蚀前后。本研究共纳入 240 个人牙本质样本，根据氟斑牙的严重程度进行分类，使用 Thylstrup-Fejerskov 指数（TFI）将其分为四组。样本在酸蚀前和酸蚀 15、30 和 60 s 后进行 AFM 分析。健康牙本质和轻度、中度、重度氟斑牙的粗糙度（R(a)）分别为 440 nm、442 nm、445 nm 和 449 nm。酸蚀 15、30 和 60 s 后，所有健康和氟斑牙本质样本的粗糙度均增加（p<0.05）。健康人牙本质的牙本质小管口（D(t)）在酸蚀 60 s 后增大。我们得出结论，健康和轻度氟斑牙本质的有效酸蚀时间为 15 s，中度氟斑牙本质为 30 s，重度氟斑牙本质为 45-60 s。
