Melnikov Olga, Baranes Nadine, Einav Monica, Ben-Dov Eitan, Manasherob Robert, Itsko Mark, Zaritsky Arieh
Department of Life Sciences, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
J Mol Microbiol Biotechnol. 2011;20(4):204-10. doi: 10.1159/000329824. Epub 2011 Jul 19.
A new gene, cry11Bb2 from a field isolate of Bacillus thuringiensis, was cloned for expression in Escherichia coli. The encoded protein, with a deduced molecular mass of 89.5 kDa, exhibits 97 and 79% identities with the overlap regions of Cry11Bb1 from B. thuringiensis ssp. medellin and Cry11Ba1 from ssp. jegathesan, respectively. It is however longer than Cry11Bb1 by 42 amino acids in its carboxy-terminus, of which 32 comprise 2 tandem repeats additional to the 5 existing in the latter polypeptide. Possible roles for this recurrent motif among Cry toxins and their accessory proteins, and for their encoding genes are proposed.
从苏云金芽孢杆菌的一个田间分离株中克隆出一个新基因cry11Bb2，并在大肠杆菌中进行表达。编码的蛋白质推导分子量为89.5 kDa，与苏云金芽孢杆菌麦德林亚种的Cry11Bb1和杰加森亚种的Cry11Ba1的重叠区域分别具有97%和79%的同一性。然而，它在羧基末端比Cry11Bb1长42个氨基酸，其中32个氨基酸构成了该多肽中除了后者已有的5个串联重复之外的另外2个串联重复。文中提出了这种重复基序在Cry毒素及其辅助蛋白中的可能作用，以及它们编码基因的可能作用。
