Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.
Food Funct. 2011 Jun;2(6):302-9. doi: 10.1039/c1fo10046g. Epub 2011 Jun 2.
Phospholipids self-assemble in bulk oils to form structures such as reverse micelles that can alter the microenvironment where chemical degradation reactions occur, such as lipid oxidation. In this study, we examined the influence of phospholipid reverse micelles on the activity of non-polar (α-tocopherol) and polar (Trolox) antioxidants in stripped soybean oil (SSO). Reverse micelles were formed by adding 1000 μM 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) to SSO. The addition of DOPC reverse micelles had a prooxidant effect, shortening the lag phase of SSO at 55 °C. DOPC improved the activity of low α-tocopherol or Trolox concentrations (10 μM) but decreased the activity of high concentrations (100 μM). Hydrophilic Trolox had better antioxidant activity than hydrophobic α-tocopherol. Fluorescence steady state and lifetime decay studies suggests that differences in the antioxidant activity of Trolox and α-tocopherol could be due to differences in their physical location in DOPC reverse micelles. These results will improve our understanding and control of lipid oxidation in bulk oils.
磷脂在大量油中自组装形成结构，如反胶束，这些结构可以改变化学降解反应（如脂质氧化）发生的微环境。在这项研究中，我们研究了磷脂反胶束对非极性（α-生育酚）和极性（Trolox）抗氧化剂在脱胶大豆油（SSO）中的活性的影响。通过向 SSO 中添加 1000μM 1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）形成反胶束。DOPC 反胶束的添加具有促氧化作用，缩短了 SSO 在 55°C 时的滞后阶段。DOPC 提高了低浓度（10μM）α-生育酚或 Trolox 的活性，但降低了高浓度（100μM）的活性。亲水性 Trolox 的抗氧化活性优于疏水性α-生育酚。荧光稳态和寿命衰减研究表明，Trolox 和α-生育酚抗氧化活性的差异可能是由于它们在 DOPC 反胶束中的物理位置不同所致。这些结果将提高我们对大量油中脂质氧化的理解和控制。
