Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece.
J Assist Reprod Genet. 2011 Sep;28(10):885-91. doi: 10.1007/s10815-011-9611-z. Epub 2011 Jul 21.
Sperm flow cytometry (SFC) was used to evaluate the association of sperm chromatin condensation and ploidy with fertilization, embryo development, pregnancy and abortion rates following IVF.
Conventional semen analysis was performed in one hundred fifty men, as well as SFC analysis, after acridine orange and propidium iodide staining, for the evaluation of sperm maturity and ploidy respectively. Conventional IVF was performed in all couples.
Couples with low percentages of mature spermatozoa presented with lower fertilization rates (p < 0.005), lower rates of grade A embryos (p < 0.003) and lower pregnancy rates (p < 0.006), compared to couples with high percentages of mature spermatozoa. Couples with low total aneuploidy rates presented with higher fertilization rates (p < 0.007), higher rates of grade A embryos (p < 0.004) and higher pregnancy rates (p < 0.003), compared to couples with high total aneuploidy rates.
Sperm chromatin condensation and ploidy constitute critical parameters for the evaluation of semen samples before IVF and for the identification of cases in need of ICSI application.
精子流式细胞术（SFC）用于评估精子染色质浓缩和倍性与体外受精后受精、胚胎发育、妊娠和流产率的关系。
对 150 名男性进行常规精液分析和吖啶橙和碘化丙啶染色后的 SFC 分析，分别评估精子成熟度和倍性。所有夫妇均进行常规体外受精。
与具有高成熟精子百分比的夫妇相比，具有低成熟精子百分比的夫妇的受精率较低（p < 0.005），A级胚胎率较低（p < 0.003），妊娠率较低（p < 0.006）。总非整倍体率较低的夫妇的受精率较高（p < 0.007），A级胚胎率较高（p < 0.004），妊娠率较高（p < 0.003）。
精子染色质浓缩和倍性是体外受精前评估精液样本和识别需要 ICSI 应用的病例的关键参数。
