Molecular characterization, tissue expression, polymorphism and association of porcine LCK gene.

Lymphocyte-specific protein tyrosine kinase (LCK) is an important reproduction related gene. In this study, the full-length cDNA sequence of porcine LCK gene was cloned through the rapid amplification of cDNA ends (RACE) method. The porcine LCK gene encodes a protein of 509 amino acids which shares high homology with the LCK of nine species: giant panda (97%), dog (97%), cattle (96%), sheep (95%), rabbit (95%), human (96%), rat (94%), mouse (94%) and horse (94%). This novel porcine gene was assigned to GeneID: 100233188. The phylogenetic analysis revealed that the porcine LCK gene has a closer genetic relationship with the LCK gene of dog. This gene is structured in twelve exons and eleven introns as revealed by computer-assisted analysis. The tissue transcription profile analysis indicated that the porcine LCK gene is generally but differentially expressed in the detected tissues including large intestine, spleen, lung, muscle, fat, liver, heart, kidney and ovary. PCR-Alu I-RFLP was established to detect an A/G substitution at the position of 1127-bp of mRNA and eight pig breeds displayed obvious genotype and allele frequency differences at this mutation locus. Association of this single-nucleotide polymorphism with litter size traits was assessed in Large White (n = 100) and Landrace (n = 100) pig populations, and results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White sows and Landrace sows (P < 0.01). Therefore, LCK gene could be an useful candidate gene in selection for increasing litter size in pigs. These data serve as a foundation for further insight into this novel porcine gene.