Programa de Pós-graduação em Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
Parasitol Res. 2012 Feb;110(2):645-8. doi: 10.1007/s00436-011-2539-5. Epub 2011 Jul 16.
The aim of the present work was to propose a new method of solubilizing thymol for use as an acaricide and to evaluate the resulting product's efficacy on Rhipicephalus microplus larvae. Two experiments were performed, both employing the larval packet test to evaluate the potential of the solutions formulated. In the first experiment, R. microplus larvae were exposed to three new formulations: formulation 1-distilled water (70%) + ethanol (30%) + thymol (10.0 mg/ml); formulation 2-distilled water (50%) + ethanol (50%) + thymol (10.0 mg/ml); and formulation 3-distilled water (55%) + ethanol (40%) + DMSO (5%) + thymol (10.0 mg/ml). A control was prepared for each formulation (same formulations except thymol), and another control group was formed where the larvae did not receive any treatment. From the results obtained in the first experiment, the formulation with the best performance and cost-benefit relation was chosen and was tested at concentrations of 2.5, 5.0 and 10.0 mg/ml on larvae of this tick. In the first experiment, the mortality of the groups treated with thymol was greater than 95% for all three formulations, with significant differences (p < 0.05) in comparison with the control groups, where the mortality was under 1%. In the second experiment, formulation 2 was selected because of its better cost-benefit relation, and the mortality rates were 94.0%, 96.5% and 99.9% for the concentrations of 2.5, 5.0 and 10.0 mg/ml, respectively, demonstrating that thymol dissolved in ethanol has high acaricide activity on cattle tick larvae.
本工作旨在提出一种新的溶解百里香酚的方法，用于杀蜱，并评估所得产物对锐缘蜱幼虫的效果。进行了两项实验，均采用幼虫包被试验来评估所配制溶液的潜力。在第一项实验中，锐缘蜱幼虫暴露于三种新配方中：配方 1-蒸馏水（70%）+乙醇（30%）+百里香酚（10.0mg/ml）；配方 2-蒸馏水（50%）+乙醇（50%）+百里香酚（10.0mg/ml）；和配方 3-蒸馏水（55%）+乙醇（40%）+DMSO（5%）+百里香酚（10.0mg/ml）。为每个配方制备了一个对照（除了百里香酚，配方相同），另一个对照组则不给幼虫任何处理。从第一项实验的结果中，选择了性能和成本效益关系最好的配方，并在该蜱幼虫的 2.5、5.0 和 10.0mg/ml 浓度下进行了测试。在第一项实验中，所有三种配方处理组的死亡率均超过 95%，与对照组（死亡率低于 1%）相比差异显著（p<0.05）。在第二项实验中，选择了配方 2，因为它具有更好的成本效益关系，浓度为 2.5、5.0 和 10.0mg/ml 时，死亡率分别为 94.0%、96.5%和 99.9%，表明溶解在乙醇中的百里香酚对牛蜱幼虫具有很高的杀蜱活性。
