Pós-Graduação em Ciências Biológicas - Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil,
Parasitol Res. 2013 Oct;112(10):3471-6. doi: 10.1007/s00436-013-3527-8. Epub 2013 Jul 11.
The objective of this study was to assess the acaricidal activity of carvacrol, thymol, eugenol, and (E)-cinnamaldehyde on unengorged larvae and nymphs of Amblyomma cajennense and Rhipicephalus sanguineus, using the modified larval packet test. Carvacrol, eugenol, and (E)-cinnamaldehyde were tested at concentrations of 2.5, 5.0, 10.0, 15.0, and 20.0 μl/ml, while thymol was tested at concentrations of 2.5, 5.0, 10.0, 15.0, and 20.0 mg/ml, in all cases with 10 repetitions per treatment. For the A. cajennense larvae, mortality rates caused by carvacrol, thymol, eugenol, and (E)-cinnamaldehyde at the lowest concentration were 45.0, 62.7, 10.2, and 81.6%, respectively, reached 100% at the concentration of 5.0 μl/ml for carvacrol and (E)-cinnamaldehyde and 5.0 mg/ml for thymol, while this mortality was observed at 15.0 μl/ml for eugenol. For the nymphs of this species, carvacrol and thymol caused 100% mortality starting at a concentration of 5.0 μl/ml and 10.0 mg/ml, respectively, while eugenol caused 100% mortality at 20.0 μl/ml and the mortality caused by (E)-cinnamaldehyde did not exceed 64%. In the tests with R. sanguineus larvae, the lowest concentration of carvacrol and (E)-cinnamaldehyde resulted in 100% mortality, while this percentage was observed starting at 10.0 μl/ml for eugenol. For nymphs, carvacrol and thymol at the smallest concentration caused 100% lethality, unlike the results for eugenol and (E)-cinnamaldehyde, where 100% mortality was only observed starting at the concentration of 10.0 μl/ml. The results obtained indicate that the tested substances have acaricidal activity on unengorged larvae and nymphs of A. cajennense and R. sanguineus.
本研究的目的是评估香芹酚、百里酚、丁香酚和（E）-肉桂醛对未饱血的安氏革蜱和血红扇头蜱幼虫和若虫的杀蜱活性，使用改良幼虫包被测试。香芹酚、丁香酚和（E）-肉桂醛的测试浓度为 2.5、5.0、10.0、15.0 和 20.0 μl/ml，而百里酚的测试浓度为 2.5、5.0、10.0、15.0 和 20.0 mg/ml，每种情况均重复 10 次。对于 A. cajennense 幼虫，香芹酚、百里酚、丁香酚和（E）-肉桂醛在最低浓度下引起的死亡率分别为 45.0%、62.7%、10.2%和 81.6%，在香芹酚和（E）-肉桂醛的 5.0 μl/ml 浓度和百里酚的 5.0 mg/ml 浓度下达到 100%，而在丁香酚的 15.0 μl/ml 浓度下观察到这种死亡率。对于该物种的若虫，香芹酚和百里酚分别在 5.0 μl/ml 和 10.0 mg/ml 的浓度下引起 100%的死亡率，而丁香酚在 20.0 μl/ml 的浓度下引起 100%的死亡率，（E）-肉桂醛引起的死亡率不超过 64%。在 R. sanguineus 幼虫的测试中，香芹酚和（E）-肉桂醛的最低浓度导致 100%的死亡率，而丁香酚在 10.0 μl/ml 的浓度下则观察到这种结果。对于若虫，香芹酚和百里酚在最小浓度下导致 100%的致死率，与丁香酚和（E）-肉桂醛的结果不同，只有在 10.0 μl/ml 的浓度下才观察到 100%的死亡率。研究结果表明，所测试的物质对未饱血的安氏革蜱和血红扇头蜱幼虫和若虫具有杀蜱活性。
