Embrapa Vegetables, Brasília, DF 70359-970, Brazil.
Arch Virol. 2011 Nov;156(11):2063-9. doi: 10.1007/s00705-011-1070-4. Epub 2011 Jul 22.
To date, no begomovirus has been fully characterized from Euphorbia heterophylla, a widely distributed weed, in Brazil. Here, we show the occurrence of a new begomovirus on E. heterophylla plants showing bright yellow mosaic. The bipartite viral genome was cloned from 10 samples, and all clones are almost identical to each other (95.6-98.8% nucleotide sequence identity). The DNA-A sequences shared a maximum nucleotide sequence identity of 87.3% with euphorbia mosaic Peru virus (EuMPV) and thus were classified as belonging to a novel begomovirus species, tentatively named Euphorbia yellow mosaic virus (EuYMV). The EuYMV DNA-B sequences share a maximum nucleotide sequence identity of 56.2% with a euphorbia mosaic virus (EuMV) isolate from Mexico. Phylogenetic analysis demonstrated that this new virus belongs to a different lineage than EuMV isolates from Central America.
迄今为止，在巴西广泛分布的杂草大飞扬草(Euphorbia heterophylla)中尚未完全鉴定出任何曲叶病毒。在这里，我们展示了一种新的曲叶病毒在表现出亮黄色花叶的大飞扬草植物上的发生。从 10 个样本中克隆出了二分体病毒基因组，所有克隆彼此几乎相同（核苷酸序列同一性为 95.6-98.8%）。DNA-A 序列与秘鲁仙客来花叶病毒(EuMPV)的最大核苷酸序列同一性为 87.3%，因此被归类为属于一种新的曲叶病毒种，暂定名为大飞扬草黄花叶病毒(EuYMV)。EuYMV DNA-B 序列与来自墨西哥的一种仙客来花叶病毒(EuMV)分离株的最大核苷酸序列同一性为 56.2%。系统发育分析表明，这种新病毒与从中美洲分离出的 EuMV 分离株属于不同的谱系。
