Toniolo C, Crisma M, Becker E L
Dipartimento di Chimica Organica Università di Padova, Italy.
Farmaco. 1990 Jul;45(7-8):921-5.
The tripeptide N alpha-carbamoyl-L-methionyl-L-leucyl-L-phenylalanine methyl ester has been synthesized in solution by classical methods and fully characterized. This compound, prepared in order to obtain a deeper insight into the mode of binding at the formyl peptide chemotactic receptor, has been tested for its ability to induce granule enzyme secretion from rabbit peritoneal neutrophils and found to be a complete agonist. These results confirm the hypothesis that a proton on the N alpha-blocking group of the tripeptide forms a hydrogen bond with an acceptor in the binding site.
三肽Nα-氨甲酰基-L-甲硫氨酰-L-亮氨酰-L-苯丙氨酸甲酯已通过经典方法在溶液中合成并得到充分表征。制备该化合物是为了更深入地了解其在甲酰肽趋化受体上的结合模式，已测试其诱导兔腹膜中性粒细胞颗粒酶分泌的能力，结果发现它是一种完全激动剂。这些结果证实了这样的假设，即三肽Nα-封闭基团上的质子与结合位点中的受体形成氢键。
