College of Biological Engineering, Jimei University, Xiamen, Fujian 361021, China.
J Sci Food Agric. 2012 Jan 30;92(2):232-8. doi: 10.1002/jsfa.4565. Epub 2011 Jul 21.
Shellfish hypersensitivity is among the most common food allergies. The allergens tropomyosin (TM) and arginine kinase (AK) from mud crab (Scylla paramamosain) were purified to homogeneity. BALB/c female mice were sensitized with TM and AK by intragastric administration. Mice treated with normal saline served as the negative control (NC) group.
Compared with NC group, mice that were treated with TM and AK developed reduced activity; meanwhile, their scratching behavior and specific-IgE level were increased. Specific-CD4 + T cells were significantly elevated in the splenocyte cultures of the mice upon TM and AK stimulation. However, compared with the positive control group (ovalbumin, OVA), there was no significant difference. The expression of IL-4 in culture cells stimulated by TM, AK, and OVA group showed significant differences from the NC group, respectively.
These results indicated that a BALB/c mouse model for sensitization to TM and AK from mud crab was successfully established, and the Th2 response was observed, displaying increased immunoglobulin E levels, together with the production of interleukin 4 and allergic symptoms.
贝类过敏是最常见的食物过敏之一。从泥蟹（锯缘青蟹）中纯化得到肌球蛋白（TM）和精氨酸激酶（AK）过敏原，达到均一性。BALB/c 雌性小鼠通过胃内给予 TM 和 AK 进行致敏。用生理盐水处理的小鼠作为阴性对照（NC）组。
与 NC 组相比，用 TM 和 AK 处理的小鼠活动减少；同时，它们的搔抓行为和特异性 IgE 水平增加。TM 和 AK 刺激后，小鼠脾细胞培养中的特异性 CD4+T 细胞显著升高。然而，与阳性对照组（卵清蛋白，OVA）相比，没有显著差异。与 NC 组相比，TM、AK 和 OVA 组培养细胞中白细胞介素 4 的表达存在显著差异。
这些结果表明，成功建立了对泥蟹 TM 和 AK 致敏的 BALB/c 小鼠模型，并观察到 Th2 反应，表现为免疫球蛋白 E 水平升高，同时产生白细胞介素 4 和过敏症状。
