Petrusz P, Van Eyck S L, Weinberg R J, Rustioni A
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Brain Res. 1990 Oct 8;529(1-2):339-44. doi: 10.1016/0006-8993(90)90848-6.
Antisera raised against glutaraldehyde conjugates of glutamate (Glu) and aspartate (Asp) with hemocyanin proved highly specific for their respective unconjugated amino acid haptens when tested in immunocytochemical blocking experiments on sections of the rat spinal cord. In addition, immunocytochemical staining by the Glu antiserum was effectively blocked by quisqualate but not by kainate or N-methyl-D-aspartate (NMDA); staining with the Asp antiserum was effectively blocked by kainate, to a lesser extent by quisqualate, and was not affected by NMDA. These results may be explained by assuming that the specific binding regions of the antibodies tested share certain recognition characteristics with endogenous binding sites or receptors for excitatory amino acids and their agonists.
在对大鼠脊髓切片进行免疫细胞化学阻断实验时，针对谷氨酸（Glu）和天冬氨酸（Asp）与血蓝蛋白的戊二醛缀合物产生的抗血清，对其各自未缀合的氨基酸半抗原表现出高度特异性。此外，谷氨酸抗血清的免疫细胞化学染色可被quisqualate有效阻断，但不能被kainate或N-甲基-D-天冬氨酸（NMDA）阻断；天冬氨酸抗血清的染色可被kainate有效阻断，被quisqualate阻断程度较小，且不受NMDA影响。这些结果可以通过假设所测试抗体的特异性结合区域与兴奋性氨基酸及其激动剂的内源性结合位点或受体具有某些识别特征来解释。
