Molecular Spectroscopy Division, Institute of Experimental Physics, University of Gdańsk, Wita Stwosza 57, 80-952 Gdańsk, Poland.
J Phys Chem B. 2011 Aug 25;115(33):10120-5. doi: 10.1021/jp202152m. Epub 2011 Aug 2.
A new method for extending the utilizable range of Förster resonance energy transfer (FRET) is proposed and tested by the Monte Carlo technique. The obtained results indicate that the efficiency of FRET can be significantly enhanced at a given distance if the energy transfer takes place toward multiple acceptors that are closely located on a macromolecule instead of a single acceptor molecule as it is currently used in FRET analysis. On the other hand, reasonable FRET efficiency can be obtained at significantly longer distances than in the case of a single acceptor. Randomly distributed and parallel orientated acceptor transition moments with respect to the transition moment of the donor molecule have been analyzed as two extreme cases. As expected, a parallel orientation of donor and acceptor transition moments results in a more efficient excitation energy transfer. This finding could be used to directly reveal the assembly/deassembly of large protein complexes in a cell by fluorescence microscopy.
提出并通过蒙特卡罗技术测试了一种扩展Förster 共振能量转移（FRET）可用范围的新方法。结果表明，如果能量转移发生在靠近大分子上的多个受体上，而不是像当前 FRET 分析中那样发生在单个受体分子上，则可以在给定距离处显著提高 FRET 的效率。另一方面，可以在比单个受体明显更长的距离处获得合理的 FRET 效率。已分析了相对于供体分子跃迁矩随机分布且平行取向的受体跃迁矩作为两种极端情况。如预期的那样，供体和受体跃迁矩的平行取向导致更有效的激发能量转移。这一发现可用于通过荧光显微镜直接揭示细胞中大型蛋白质复合物的组装/解组装。
