TC21/R-ras2 在高度侵袭性恶性皮肤癌中表达更高。

Greater expression of TC21/R-ras2 in highly aggressive malignant skin cancer.

机构信息

Department of Plastic and Reconstructive Surgery, Hanyang University Guri Hospital, Guri, Korea.

出版信息

Int J Dermatol. 2011 Aug;50(8):956-60. doi: 10.1111/j.1365-4632.2010.04846.x.

DOI:10.1111/j.1365-4632.2010.04846.x

PMID:21781067

Abstract

BACKGROUND

TC21 plays an important role in highly aggressive tumor formation, and it was overexpressed in several human cancers, including breast cancer, oral squamous cell carcinoma (SCC), and esophageal SCC. In light of this, we explored the expression of TC21 in overall skin cancers in order to evaluate the relationship between TC21 and malignant skin tumors.

METHODS

We examined six normal skin tissues and 18 malignant skin tumor tissues, including six malignant melanomas (MM), six SCCs, and six basal cell carcinomas (BCCs) using western blotting for the expression of TC21. In another set, 16 specimens of MM, 16 SCC, and 16 BCC were analyzed for the expression of TC21 using immunohistochemical staining. To evaluate the amount of expression of TC21, the Raytest TINA software was used for western blotting and a histochemical score (HSCORE) was used for immunohistochemical evaluation.

RESULTS

The western blotting and immunohistochemistry showed that TC21 was expressed in all malignant skin tumors and not in normal skin tissues. The relative protein expression was an average of 0.004 in normal skin, 1.042 in MM, 0.621 in SCC, and 0.485 in BCC. In immunohistochemistry, HSCORE for normal skin was an average of 0.05, MM was 2.42, SCC was 2.11, and BCC was 1.22.

CONCLUSIONS

This article is the first study demonstrating expression of TC21 in human skin malignant tumors and suggests that TC21 is more expressed in highly aggressive skin tumors.

摘要

背景

TC21 在高度侵袭性肿瘤形成中发挥重要作用，它在包括乳腺癌、口腔鳞状细胞癌（SCC）和食管 SCC 在内的几种人类癌症中过度表达。有鉴于此，我们探索了 TC21 在所有皮肤癌中的表达，以评估 TC21 与恶性皮肤肿瘤之间的关系。

方法

我们使用 Western blot 检查了 6 个正常皮肤组织和 18 个恶性皮肤肿瘤组织，包括 6 个恶性黑色素瘤（MM）、6 个 SCC 和 6 个基底细胞癌（BCC），以检测 TC21 的表达。在另一组中，我们使用免疫组织化学染色分析了 16 个 MM、16 个 SCC 和 16 个 BCC 标本中 TC21 的表达。为了评估 TC21 的表达量，我们使用 Raytest TINA 软件进行 Western blot 分析，并使用组织化学评分（HSCORE）进行免疫组织化学评估。

结果

Western blot 和免疫组化显示 TC21 在所有恶性皮肤肿瘤中表达，而在正常皮肤组织中不表达。相对蛋白表达在正常皮肤中平均为 0.004，在 MM 中为 1.042，在 SCC 中为 0.621，在 BCC 中为 0.485。在免疫组化中，正常皮肤的 HSCORE 平均为 0.05，MM 为 2.42，SCC 为 2.11，BCC 为 1.22。