[RNA干扰调控Y盒蛋白1对阿霉素诱导K562细胞中mdr1基因表达的影响]

[Effect of regulation of Y-box protein 1 by RNA interference on the doxorubicin induced mdr1 gene expression in K562 cells].

作者信息

Shen Hui-Ling, Xu Wen-Lin, Chen Qiao-Yun, Wang Fa-Chun, Fei Xia

机构信息

Department of Central Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2011 Jun;32(6):383-7.

PMID:21781495

Abstract

OBJECTIVE

To investigate the effect of YB-1 on the transcription of induced mdr1 gene expression in K562 cells.

METHODS

K562 cells were treated with doxorubicin (DOX) at different concentrations and times. Expression of mdr1 and YB-1 genes was examined by RT-PCR and P-glycoprotein (P-gp) by flow cytometry. Cyto/nuclear protein was extracted for YB-1 detection by Western blotting. The expression of YB-1 gene in K562 cells was inhibited by YB-1 gene specific RNA interference (RNAi), then the expression of mdr1 and P-gp in YB-1 gene silenced cells treated with DOX was detected.

RESULTS

The mdr1 gene as well as its corresponding protein P-gp was highly expressed in DOX exposed K562 cells. DOX up-regulated the expression of YB-1 gene, and promoted YB-1 protein nuclear translocation. On YB-1 gene silenced, the expressions of mdr1 gene and P-gp were obviously down-regulated in DOX treated K562 cells.

CONCLUSION

Doxorubicin can induce the expression of mdr1 gene in K562 cells, which may result from the transcription of mdr1 gene by activated YB-1.

摘要

目的

研究YB-1对K562细胞中诱导的mdr1基因表达转录的影响。

方法

用不同浓度和时间的阿霉素（DOX）处理K562细胞。通过RT-PCR检测mdr1和YB-1基因的表达，通过流式细胞术检测P-糖蛋白（P-gp）。提取细胞/核蛋白，通过蛋白质印迹法检测YB-1。通过YB-1基因特异性RNA干扰（RNAi）抑制K562细胞中YB-1基因的表达，然后检测用DOX处理的YB-1基因沉默细胞中mdr1和P-gp的表达。