Shen Hui-Ling, Xu Wen-Lin, Chen Qiao-Yun, Wang Fa-Chun, Fei Xia
Department of Central Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China.
Zhonghua Xue Ye Xue Za Zhi. 2011 Jun;32(6):383-7.
To investigate the effect of YB-1 on the transcription of induced mdr1 gene expression in K562 cells.
K562 cells were treated with doxorubicin (DOX) at different concentrations and times. Expression of mdr1 and YB-1 genes was examined by RT-PCR and P-glycoprotein (P-gp) by flow cytometry. Cyto/nuclear protein was extracted for YB-1 detection by Western blotting. The expression of YB-1 gene in K562 cells was inhibited by YB-1 gene specific RNA interference (RNAi), then the expression of mdr1 and P-gp in YB-1 gene silenced cells treated with DOX was detected.
The mdr1 gene as well as its corresponding protein P-gp was highly expressed in DOX exposed K562 cells. DOX up-regulated the expression of YB-1 gene, and promoted YB-1 protein nuclear translocation. On YB-1 gene silenced, the expressions of mdr1 gene and P-gp were obviously down-regulated in DOX treated K562 cells.
Doxorubicin can induce the expression of mdr1 gene in K562 cells, which may result from the transcription of mdr1 gene by activated YB-1.
研究YB-1对K562细胞中诱导的mdr1基因表达转录的影响。
用不同浓度和时间的阿霉素（DOX）处理K562细胞。通过RT-PCR检测mdr1和YB-1基因的表达，通过流式细胞术检测P-糖蛋白（P-gp）。提取细胞/核蛋白，通过蛋白质印迹法检测YB-1。通过YB-1基因特异性RNA干扰（RNAi）抑制K562细胞中YB-1基因的表达，然后检测用DOX处理的YB-1基因沉默细胞中mdr1和P-gp的表达。
在暴露于DOX的K562细胞中，mdr1基因及其相应蛋白P-gp高度表达。DOX上调YB-1基因的表达，并促进YB-1蛋白的核转位。在YB-1基因沉默后，DOX处理的K562细胞中mdr1基因和P-gp的表达明显下调。
阿霉素可诱导K562细胞中mdr1基因的表达，这可能是由于激活的YB-1对mdr1基因的转录所致。
