[哮喘患者诱导痰中气道炎症与基质细胞衍生因子1水平的相关性]

[Correlation between the airway inflammations and levels of stromal cell-derived factor 1 in induced sputum of asthmatic patients].

作者信息

Wu Qiu-ge, Wang Jing, Zhou Shi-xia

机构信息

Department of Respiratory and Sleep Disease, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2011 Jun;34(6):429-32.

PMID:21781514

Abstract

OBJECTIVE

To evaluate concentrations of stromal cell-derived factor 1 (SDF-1) and IL-17 in induced sputum supernatants from asthmatic patients before and after treatment with glucocorticosteroids.

METHODS

Induced sputum was collected from 30 healthy controls and 99 patients with chronic persistent asthma from 2009-2010. Sputum samples were obtained before and after 4 week treatment with inhaled glucocorticosteroids. The sputum concentrations of SDF-1 and IL-17 were measured by ELISA.

RESULTS

The FEV(1)% and the asthma control score of patients with severe asthma were decreased as compared with patients with moderate persistent and mild persistent asthma (F = 457.448 and 79.271, all P < 0.01). The concentrations of SDF-1, IL-17 and the percentage of eosinophils were increased in asthma group compared with control subjects (all P < 0.01), but the percentage of sputum neutrophils was lower than that in the healthy controls (P < 0.01). The percentage of sputum neutrophils and eosinophils and the level of SDF-1 and IL-17 in patients with severe persistent asthma were significantly higher than those in patients with mild persistent asthma (all P < 0.05). The percentage of sputum neutrophils and eosinophils were negatively correlated with FEV(1)% (r = -0.409 and -0.316, all P < 0.05). The levels of IL-17 and SDF-1 were positively correlated with the percentage of sputum neutrophils and eosinophils (all P < 0.01). The levels of IL-17 were positively correlated with the levels of SDF-1 (r = 0.872, P < 0.01). After glucocorticosteroid therapy, the percentage of eosinophils and neutrophils, the levels of IL-17 and SDF-1 decreased significantly in all patients (all P < 0.01), while the percentage of sputum neutrophils and the levels of IL-17 and SDF-1 in uncontrolled patients increased significantly compared with the controlled and partly controlled groups (all P < 0.05).

CONCLUSIONS

SDF-1 and IL-17 may contribute to airway inflammation in asthma by chemotactic activity towards neutrophils. The concentration of SDF-1 may be used to evaluate the inflammation and the therapeutic effects.

摘要

目的

评估糖皮质激素治疗前后哮喘患者诱导痰上清液中基质细胞衍生因子1（SDF-1）和白细胞介素17（IL-17）的浓度。

方法

收集2009年至2010年30名健康对照者和99例慢性持续性哮喘患者的诱导痰。在吸入糖皮质激素治疗4周前后获取痰样本。采用酶联免疫吸附测定法（ELISA）检测痰中SDF-1和IL-17的浓度。

结果

重度哮喘患者的第1秒用力呼气容积百分比（FEV(1)%）和哮喘控制评分低于中度持续性和轻度持续性哮喘患者（F = 457.448和79.271，均P < 0.01）。与对照组相比，哮喘组SDF-1、IL-17浓度及嗜酸性粒细胞百分比升高（均P < 0.01），但痰中性粒细胞百分比低于健康对照组（P < 0.01）。重度持续性哮喘患者痰中性粒细胞和嗜酸性粒细胞百分比以及SDF-1和IL-17水平显著高于轻度持续性哮喘患者（均P < 0.05）。痰中性粒细胞和嗜酸性粒细胞百分比与FEV(1)%呈负相关（r = -0.409和-0.316，均P < 0.05）。IL-17和SDF-1水平与痰中性粒细胞和嗜酸性粒细胞百分比呈正相关（均P < 0.01）。IL-17水平与SDF-1水平呈正相关（r = 0.872，P < 0.01）。糖皮质激素治疗后，所有患者嗜酸性粒细胞和中性粒细胞百分比、IL-17和SDF-1水平均显著降低（均P < 0.01），而未控制患者的痰中性粒细胞百分比以及IL-17和SDF-1水平与控制组和部分控制组相比显著升高（均P < 0.05）。