[从日本水杨梅中分离出的一种新成分对梗死心脏的心肌再生与修复作用]

[Myocardial regeneration and repair of infarcted heart by a new composition isolated from Geum japonicum].

作者信息

Mo Ye-hua, Peng Peng, Lin Xiao-li, Li Zhong-yu, Cheng Lei, Li Ming

机构信息

Laboratory of Regenerative Medicine, Jinan University, Guangzhou 510632, China.

出版信息

Zhonghua Xin Xue Guan Bing Za Zhi. 2011 May;39(5):414-9.

PMID:21781595

Abstract

OBJECTIVES

To isolate the cardiogenic fraction, which can enhance cardiogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC) from Geum japonicum. The therapeutic effect of the isolated cardiogenic fraction was further tested in a rat myocardial infarction (MI) model.

METHOD

Bioassay guided fractionation method was used for the isolation of the cardiogenic fraction, named as heart repair fraction (HRF). MI was induced by a permanent ligation of left anterior descending coronary artery. The rats exhibiting similarly decreased values of left ventricle ejection fraction (LVEF) and fraction shortening (LVFS) were used. The rats in test group (n = 10) were subject to HRF treatment (20 mg×kg(-1)×d(-1)) through gastric gavage daily for 4 weeks. Water alone (2 ml/d) was given through gastric gavage to rats in the control group (n = 10). The cardiac function was assessed by echocardiography at different time points. Masson trichrome staining was used for evaluation of the infarct size. Morphological and immunohistochemical studies were performed to investigate the HRF mediated myocardial regeneration.

RESULTS

LVEF (66.2% ± 6.9%) and LVFS (46.8% ± 5.8%) were significantly increased two weeks post HRF treatment compared with the values (LVEF: 55.7% ± 6.0% and LVFS: 36.4% ± 5.2%) in control rats (all P < 0.01). The improved heart function was further restored 4 weeks post HRF treatment (P < 0.01). Furthermore, the treatment of acute MI with this HRF significantly reduced the infarct size (19.0% ± 6.1%) compared with that (31.1% ± 8.6%) in control rats (P < 0.01). Substantial regeneration of cardiomyocytes in infarcted region of the HRF treated heart was also observed that replaced a considerable part of the infarcted heart tissues resulting in remarkable reduction of the infarct size.

CONCLUSION

The properties of this HRF isolated from Geum japonicum in stimulating substantial regeneration of myocardium in infarct region with consequently improved cardiac function appear to be new and represent a new approach for the treatment of MI.

摘要

目的

从日本水杨梅中分离出可增强骨髓间充质干细胞（MSC）向心肌细胞分化的心肌源性组分。在大鼠心肌梗死（MI）模型中进一步测试分离出的心肌源性组分的治疗效果。

方法

采用生物测定引导分级分离法分离出心肌源性组分，命名为心脏修复组分（HRF）。通过永久性结扎左冠状动脉前降支诱导心肌梗死。选用左心室射血分数（LVEF）和缩短分数（LVFS）值同样降低的大鼠。试验组（n = 10）大鼠每天经胃管给予HRF治疗（20 mg×kg⁻¹×d⁻¹），持续4周。对照组（n = 10）大鼠经胃管单独给予水（2 ml/d）。在不同时间点通过超声心动图评估心脏功能。采用Masson三色染色法评估梗死面积。进行形态学和免疫组织化学研究以探究HRF介导的心肌再生。

结果

与对照组大鼠（LVEF：55.7% ± 6.0%，LVFS：36.4% ± 5.2%）相比，HRF治疗后两周LVEF（66.2% ± 6.9%）和LVFS（46.8% ± 5.8%）显著升高（均P < 0.01）。HRF治疗4周后心脏功能的改善进一步恢复（P < 0.01）。此外，与对照组大鼠（31.1% ± 8.6%）相比，用该HRF治疗急性心肌梗死可显著减小梗死面积（19.0% ± 6.1%）（P < 0.01）。在HRF治疗的心脏梗死区域还观察到心肌细胞大量再生，取代了相当一部分梗死心脏组织，从而显著减小了梗死面积。