人类对刚地弓形虫核苷三磷酸水解酶的抗体反应。

Human antibody response to the nucleoside triphosphate hydrolase of Toxoplasma gondii.

作者信息

Tenter A M, Johnson A M

机构信息

Department of Clinical Microbiology, Flinders University School of Medicine, Flinders Medical Centre, South Australia.

出版信息

J Immunoassay. 1990;11(4):579-90. doi: 10.1080/01971529008055051.

DOI:10.1080/01971529008055051

PMID:2178171

Abstract

An enzyme-linked immunosorbent assay (ELISA) that uses a recombinant protein of Toxoplasma gondii as antigen was used for the detection of specific antibodies in human sera. An antigenic portion of the T. gondii nucleoside triphosphate hydrolase (NTPase) was expressed in Escherichia coli as a glutathione S-transferase fusion protein with an apparent molecular mass of about 5000. A total of 118 T. gondii positive and 63 negative sera were examined. Seven % of the T. gondii positive sera, but none of the negative sera, reacted with the recombinant NTPase. Advantages and disadvantages that are associated with the use of fusion proteins as antigens in ELISA are discussed.

摘要

一种以刚地弓形虫重组蛋白为抗原的酶联免疫吸附测定（ELISA）用于检测人血清中的特异性抗体。刚地弓形虫核苷三磷酸水解酶（NTPase）的一个抗原部分在大肠杆菌中表达为谷胱甘肽S-转移酶融合蛋白，表观分子量约为5000。共检测了118份刚地弓形虫阳性血清和63份阴性血清。7%的刚地弓形虫阳性血清与重组NTPase发生反应，而阴性血清均未发生反应。文中讨论了在ELISA中使用融合蛋白作为抗原的优缺点。