Arkansas Cancer Research Center, Little Rock, AR 72205, USA.
Environ Toxicol Pharmacol. 1997 Dec;4(3-4):277-81. doi: 10.1016/s1382-6689(97)10023-0.
Gene-environment interaction is an important aspect of human cancer risk. Genetic polymorphisms in acetylation and N-oxidation have previously been described regarding their impact on the heterocyclic amine-induced risk for colon cancer. Here, we report that another enzyme involved in the metabolism of food-borne carcinogens, sulfotransferase (ST1A3 measured by 2-naphthol activity), may function as a potential protective factor for colon cancer in humans. Initially characterized in human liver and colon (Chou et al., 1995), TS-PST activity can also be measured in platelets. A simple microtiter-based colorimetric technique was developed for use in this case-control study. African-Americans had a higher mean ST activity than Caucasians (2.32±0.24 versus 1.77±0.09 nmols/min per mg cytosolic protein, P=0.036). Furthermore, the slow ST phenotype (ST≤1.53) was more frequently associated with colon cancer than controls (57 versus 40%, P=0.026). These data suggest that the ST1A3 isoform may play a role in the differential risk for colorectal cancer.
基因-环境相互作用是人类癌症风险的一个重要方面。先前已经描述了乙酰化和 N-氧化的遗传多态性，它们对杂环胺诱导结肠癌风险的影响。在这里，我们报告另一种参与食物源性致癌物质代谢的酶，磺基转移酶（通过 2-萘酚活性测量的 ST1A3），可能是人类结肠癌的潜在保护因素。该酶最初在人类肝脏和结肠中被表征（Chou 等人，1995 年），TS-PST 活性也可以在血小板中测量。为此病例对照研究开发了一种简单的基于微孔板的比色技术。非裔美国人的 ST 活性平均值高于白种人（2.32±0.24 与 1.77±0.09nmols/min per mg 胞质蛋白，P=0.036）。此外，与对照组相比，缓慢的 ST 表型（ST≤1.53）更频繁地与结肠癌相关（57%比 40%，P=0.026）。这些数据表明，ST1A3 同工酶可能在结直肠癌的不同风险中发挥作用。
