Department of Physiology and Pathology, School of Dentistry, São Paulo State University, UNESP, Araraquara 14801-903, São Paulo, Brazil.
Physiol Behav. 2011 Oct 24;104(5):659-65. doi: 10.1016/j.physbeh.2011.07.010. Epub 2011 Jul 18.
Gavage of 2 M NaCl (IG 2 M NaCl), a procedure to induce cell-dehydration-and water and 0.15 M NaCl intake in a two-bottle choice test-is also a potential gastric irritant. In this study, we assessed whether mineral intake induced by IG 2 M NaCl is associated with gastric irritation or production of pica in the rat. We first determined the amount of mineral solution (0.15 M NaCl, 0.15 M NaHCO3, 0.01 M KCl and 0.05 mM CaCl2) and water ingested in response to IG 2 M NaCl in a five-bottle test. Then, we used mineral solutions (0.01 M KCl and 0.15 M NaHCO3), whose intakes were significantly increased compared to controls, and water in three-bottle tests to test the gastric irritation hypothesis. The IG 2 M NaCl induced KCl and NaHCO3 intake that was not inhibited by gavage with gastric protectors Al(OH)3 or NaHCO3. IG 2 M NaCl or gavage of 0.6 N acetic acid induced mild irritation, hyperemia, of the glandular part of the stomach. A gavage of 50% ethanol induced strong irritation seen as pinpoint ulcerations. Neither ethanol nor acetic acid induced any fluid intake. Neither IG 2 M NaCl nor acetic acid induced kaolin intake, a marker of pica in laboratory rats. Ethanol did induce kaolin intake. These results suggest that IG 2 M NaCl induced a mineral fluid intake not selective for sodium and independent from gastric irritation or pica.
灌胃 2 M NaCl（IG 2 M NaCl），一种诱导细胞脱水和水以及 0.15 M NaCl 摄入的程序，也是一种潜在的胃刺激性物质。在这项研究中，我们评估了 IG 2 M NaCl 诱导的矿物质摄入是否与大鼠的胃刺激或异食癖有关。我们首先在五瓶测试中确定了对 IG 2 M NaCl 反应的矿物质溶液（0.15 M NaCl、0.15 M NaHCO3、0.01 M KCl 和 0.05 mM CaCl2）和水的摄入量。然后，我们使用了矿物质溶液（0.01 M KCl 和 0.15 M NaHCO3），与对照相比，其摄入量显著增加，并在三瓶测试中使用水来测试胃刺激假说。IG 2 M NaCl 诱导的 KCl 和 NaHCO3 摄入不受胃保护剂 Al(OH)3 或 NaHCO3 灌胃的抑制。IG 2 M NaCl 或 0.6 N 乙酸灌胃引起胃腺部分轻度刺激、充血。50%乙醇灌胃引起强烈刺激，表现为针尖状溃疡。乙醇和乙酸都不会引起任何液体摄入。IG 2 M NaCl 或乙酸都不会引起高岭土摄入，高岭土是实验室大鼠异食癖的标志物。乙醇确实会引起高岭土摄入。这些结果表明，IG 2 M NaCl 诱导的矿物质液摄入不是选择性的钠摄入，也不依赖于胃刺激或异食癖。
