Peacock J W, Matlashewski G J, Benchimol S
Ontario Cancer Institute, Toronto, Canada.
Oncogene. 1990 Dec;5(12):1769-74.
A number of cellular and viral genes encode proteins that play a role in the establishment of normal cells in culture. In addition, these genes cooperate with activated ras genes to induce cellular transformation. We show that ras-dependent transformation of rat embryo fibroblasts is more efficient when two establishment genes are used together compared with one alone. Both quantitative and qualitative differences in the efficiency of transformation were detected. The number of transformed foci generated was greater than the sum of the foci obtained with ras and each of the establishment genes used separately. In addition, the foci had a distinct morphology. Synergism was seen between the HPV-16 E7 gene and certain mutant alleles of the cellular p53 gene as well as between E7 and c-myc.
许多细胞基因和病毒基因编码的蛋白质在培养的正常细胞的建立过程中发挥作用。此外，这些基因与激活的ras基因协同作用以诱导细胞转化。我们发现，与单独使用一个建立基因相比，同时使用两个建立基因时，大鼠胚胎成纤维细胞的ras依赖性转化效率更高。在转化效率上检测到了数量和质量上的差异。产生的转化灶数量大于分别用ras和每个建立基因获得的灶数之和。此外，这些灶具有独特的形态。在人乳头瘤病毒16型E7基因与细胞p53基因的某些突变等位基因之间以及E7与c-myc之间观察到了协同作用。
