黄芩与清暑益气汤联用改善荷瘤化疗小鼠恶病质症状和免疫功能。

Improving cachectic symptoms and immune strength of tumour-bearing mice in chemotherapy by a combination of Scutellaria baicalensis and Qing-Shu-Yi-Qi-Tang.

机构信息

Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.

出版信息

Eur J Cancer. 2012 May;48(7):1074-84. doi: 10.1016/j.ejca.2011.06.048. Epub 2011 Jul 23.

DOI:10.1016/j.ejca.2011.06.048

PMID:21782419

Abstract

BACKGROUND

Cancer cachexia is characterised by the loss of body mass and directly compromises immune response and the quality of life of cancer patients. In the present study, we set out to investigate the role of Chinese herbs as anticancer medicines and/or chemotherapeutic adjuvants to increase therapeutic efficacy and/or ameliorate given side-effects in animal model.

METHODS

Twelve kinds of herbs were chosen from the ingredients of major Chinese herbal medicines, and their effects on the antioxidant activity were investigated. To obtain the anticancer effects of 5-fluorouracil (5-FU) when consumed with minimal side-effects, we investigated the combination effect of Scutellaria baicalensis and Qing-Shu-Yi-Qi-Tang that may enhance the anticancer activity of 5-FU on subcutaneous tumour growth in C57BL/6 mice challenged with Lewis lung carcinoma cells.

RESULTS

Qing-Shu-Yi-Qi-Tang, a multiple-component herbal extract, was shown to have high anti-oxidation activity, while S. baicalensis (Chinese skullcap) was demonstrated to have high tumour-growth inhibition activity. Thus, S. baicalensis and Qing-Shu-Yi-Qi-Tang were evaluated for their combinaton effects on the cancer-induced cachectic murine upon receiving 5-FU chemotherapy. As a result, tumour masses and losses of carcass and/or gastrocnemius muscle were found to be significantly decreased. This combination otherwise increased both Th1/Th2 ratio and NK cytotoxicity. In the mice receiving with or without 5-FU, the serum levels of monocyte chemoattractant protein-1 (MCP-1) increased by all means but otherwise decreased when the herbal combination was administrated. Additionally, the expressions of nuclear factor-kappa B (NF-κB) and muscle RING finger protein-1 (MuRF-1) decreased in the gastrocnemius muscle when the herbal combination was applied.

CONCLUSION

Our results revealed that the combination of S. baicalensis and Qing-Shu-Yi-Qi-Tang is able to ameliorate cachectic symptoms and positively stimulate anti-tumour immunity while undergoing chemotherapy in animal model.

摘要

背景

癌症恶病质的特征是体重减轻，直接影响免疫反应和癌症患者的生活质量。在本研究中，我们旨在研究中草药作为抗癌药物和/或化疗辅助药物的作用，以提高治疗效果和/或改善动物模型中的给定副作用。

方法

从主要中草药成分中选择了 12 种草药，研究其抗氧化活性。为了在使用最小副作用的情况下获得 5-氟尿嘧啶（5-FU）的抗癌效果，我们研究了黄芩和清气益气汤的组合效应，该组合可能增强 5-FU 对 C57BL/6 小鼠皮下肿瘤生长的抗癌活性，这些小鼠受到 Lewis 肺癌细胞的挑战。

结果

清气益气汤是一种多成分草药提取物，具有很高的抗氧化活性，而黄芩（黄芩）则表现出很高的肿瘤生长抑制活性。因此，评估了黄芩和清气益气汤对接受 5-FU 化疗的癌症诱发恶病质小鼠的组合效果。结果发现，肿瘤质量以及胴体和/或比目鱼肌的损失明显减少。这种组合还增加了 Th1/Th2 比值和 NK 细胞毒性。在接受或不接受 5-FU 的小鼠中，血清单核细胞趋化蛋白-1（MCP-1）水平均升高，但当给予草药组合时则降低。此外，当应用草药组合时，腓肠肌中的核因子-κB（NF-κB）和肌肉环指蛋白-1（MuRF-1）的表达减少。