Faculty of Medicine, Institute of Bioanalysis, University of Pécs, 12 Szigeti Str., 7633 Pécs, Hungary.
Environ Toxicol Pharmacol. 2004 Apr;16(3):163-8. doi: 10.1016/j.etap.2003.12.003.
Differential scanning calorimetry (DSC) and electron paramagnetic resonance spectroscopy (EPR) experiments were performed on human erythrocyte membranes and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) model systems in order to study the effect the polycyclic aromatic hydrocarbons on lipid structure and dynamics. Eight different compounds among others naphthalene and pyrene were compared, which occur in significant concentrations in dust collected from the air in large cities. Experiments using spin label technique showed that the compounds induced mobility changes in the lipid region in the environment of the fatty acid probe molecules incorporated into the membranes. The effects depended on the structure and concentration of the different compounds. Similarly to EPR observations, DSC measurements reported decrease of transition temperature in comparison to control DPPC vesicles. These results suggest that polycyclic aromatic hydrocarbons were able to modify the internal dynamics of erythrocyte membranes which might lead to damage of the biological functions.
为了研究多环芳烃对脂质结构和动力学的影响，我们对人红细胞膜和 1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）模型系统进行了差示扫描量热法（DSC）和电子顺磁共振波谱（EPR）实验。实验中比较了 8 种不同的化合物，包括萘和芘，这些化合物在从大城市空气中收集的灰尘中浓度很高。使用自旋标记技术的实验表明，这些化合物诱导了脂质区域在脂肪酸探针分子掺入膜中的环境中的迁移变化。这些影响取决于不同化合物的结构和浓度。与 EPR 观察结果类似，DSC 测量结果表明与对照 DPPC 囊泡相比，转变温度降低。这些结果表明，多环芳烃能够改变红细胞膜的内部动力学，这可能导致生物功能受损。
