Wadhwani Research Centre, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, India.
Int J Biol Macromol. 2011 Nov 1;49(4):737-41. doi: 10.1016/j.ijbiomac.2011.07.008. Epub 2011 Jul 19.
The assembly of FtsZ plays an important role in bacterial cell division. Lipids in the bacterial cell membrane have been suggested to play a role in directing the site of FtsZ assembly. Using lipid monolayer and bilayer (liposome) systems, we directly examined the effects of cationic lipids on FtsZ assembly. We found that cationic lipids enhanced the assembly of FtsZ in association with an increase in the GTPase activity of FtsZ. The system consisting of lipid monolayer and bilayer (liposome) may mimic the bacterial membrane and therefore, the data might indicate the influence of bacterial membrane on the assembly of FtsZ protofilaments.
FtsZ 的组装在细菌细胞分裂中起着重要作用。有人提出，细菌细胞膜中的脂质在指导 FtsZ 组装的位置方面发挥了作用。使用单层脂质和双层脂质体（liposome）系统，我们直接研究了阳离子脂质对 FtsZ 组装的影响。我们发现，阳离子脂质增强了 FtsZ 的组装，同时 FtsZ 的 GTPase 活性也增加。由单层脂质和双层脂质体（liposome）组成的系统可能模拟了细菌膜，因此，这些数据可能表明了细菌膜对 FtsZ 原丝组装的影响。
