Department of Pediatrics, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, USA.
Neurosci Lett. 2011 Sep 1;501(3):148-51. doi: 10.1016/j.neulet.2011.06.062. Epub 2011 Jul 18.
The inflammatory response following traumatic injury to the central nervous system (CNS) includes the infiltration of large numbers of macrophages. This response has been implicated in both ongoing tissue damage as well as recovery following CNS injury. We investigated the role of invading macrophages on one important aspect of tissue repair in the brain, the reformation of the blood brain barrier (BBB). We used liposomal clodronate to deplete monocytes and tissue macrophages. This method led to a marked reduction in the accumulation of F4/80-expressing cells at sites of hypothermic brain injury in a murine model. The integrity of the blood brain barrier over time following injury was assessed by permeability of fluorescent labeled albumin. The reduction in macrophages at the injury site was accompanied by a delay in early reformation of the blood brain barrier. In control animals the permeability of the BBB to FITC-labeled albumin returned to normal levels by seven days post-injury. In macrophage-depleted mice leakage of albumin was still observed at seven days post-injury. These results suggest that macrophages play an important role in early post-traumatic reformation of the BBB.
创伤性中枢神经系统（CNS）损伤后的炎症反应包括大量巨噬细胞的浸润。这种反应与 CNS 损伤后的持续组织损伤以及恢复都有关。我们研究了浸润巨噬细胞在大脑组织修复的一个重要方面——血脑屏障（BBB）重建中的作用。我们使用脂质体氯膦酸盐来耗竭单核细胞和组织巨噬细胞。这种方法导致在低温脑损伤的小鼠模型中，F4/80 表达细胞在损伤部位的积累明显减少。损伤后血脑屏障的完整性随时间通过荧光标记白蛋白的通透性进行评估。损伤部位巨噬细胞的减少伴随着血脑屏障早期重建的延迟。在对照动物中，FITC 标记白蛋白的 BBB 通透性在损伤后 7 天恢复正常水平。在巨噬细胞耗竭的小鼠中，在损伤后 7 天仍观察到白蛋白渗漏。这些结果表明，巨噬细胞在创伤后早期 BBB 重建中发挥重要作用。
