Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland.
Mitochondrion. 2012 Jan;12(1):144-8. doi: 10.1016/j.mito.2011.06.015. Epub 2011 Jul 20.
Myogenesis is accompanied by an intensive metabolic remodeling. We investigated the mitochondrial reactive oxygen species (ROS) generation at different levels of skeletal muscle differentiation: in C2C12 myoblasts, in C2C12 myotubes and in adult mouse skeletal muscle. Differentiation was accompanied by an increase in mitochondrial content and respiratory chain activity. The detected ROS production levels correlated with mitochondrial content, being the lowest in the myoblasts. Unlike the adult skeletal muscle, myoblast ROS production was significantly stimulated by the complex I inhibitor rotenone. Our results show that mitochondria are an important ROS source in skeletal muscle cells. The substantial changes in mitochondrial ROS synthesis during skeletal muscle differentiation can be explained by intensive bioenergetic remodeling.
成肌作用伴随着剧烈的代谢重塑。我们研究了不同分化阶段的骨骼肌中线粒体活性氧（ROS）的生成：在 C2C12 成肌细胞、C2C12 肌管和成年小鼠骨骼肌中。分化伴随着线粒体含量和呼吸链活性的增加。检测到的 ROS 产生水平与线粒体含量相关，在成肌细胞中最低。与成年骨骼肌不同，成肌细胞的 ROS 产生被复合物 I 抑制剂鱼藤酮显著刺激。我们的结果表明，线粒体是骨骼肌细胞中重要的 ROS 来源。在骨骼肌分化过程中，线粒体 ROS 合成的显著变化可以通过剧烈的生物能量重塑来解释。
