Innovative use of Light and Materials/Life, JST-PRESTO, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
Bioorg Med Chem Lett. 2011 Sep 15;21(18):5457-9. doi: 10.1016/j.bmcl.2011.06.119. Epub 2011 Jul 7.
Photoresponsive 5'-caps that can be reversibly cis-trans isomerized by light irradiation were developed for the reversible photoregulation of gene expression. The 8-naphthylvinyl cap (8NV-cap) in the trans form completely inhibited translation of mRNA, whereas the cis form yielded protein with the same efficiency as mRNA capped with the normal-cap, a 26-fold higher efficiency than that of the trans form. The 8NV-capped mRNA could be switched between a translating state (ON state) and a non-translating state (OFF state) in a reversible fashion by alternately irradiating with monochromatic 410 nm or 310 nm light.
为了实现基因表达的可逆光调控，开发了可通过光照进行可逆顺反异构化的光响应 5'帽。反式 8-萘乙烯基帽（8NV-cap）完全抑制 mRNA 的翻译，而顺式帽的翻译效率与用正常帽加帽的 mRNA 相同，比反式帽的翻译效率高 26 倍。通过交替用单色 410nm 或 310nm 光照射，8NV 帽mRNA 可以可逆地在翻译状态（ON 状态）和非翻译状态（OFF 状态）之间切换。
