Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.
Environ Toxicol Pharmacol. 2005 Jul;20(1):1-5. doi: 10.1016/j.etap.2004.09.009. Epub 2004 Nov 24.
Normal lymphocytes are highly sensitive to the damaging effect of radiation and undergo cell death. In the present study, the photoprotective effect of sesamol, a constituent of sesame oil, has been examined in the UVB-(280-320nm) irradiated human blood lymphocytes. Lymphocytes pretreated with increasing concentrations of sesamol (1, 5 and 10μg/ml) for 30min, were irradiated and lipid peroxidation and antioxidant defense were examined. UVB-irradiated lymphocytes exhibited increased levels of lipid peroxidation and disturbances in antioxidant defense. Sesamol pretreatment resulted in significant reduction in lipid peroxidation marker, thiobarbituric acid reactive substances (TBARS). Further, antioxidants like reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) increased, in a dose-dependent manner, in sesamol pretreated and UVB-irradiated lymphocytes. The maximum dose of sesamol (10μg/ml) normalized the UVB induced lipid peroxidation, indicating the photoprotective effect of sesamol in irradiated lymphocytes.
正常淋巴细胞对辐射的损伤作用极为敏感，会发生细胞死亡。在本研究中，我们研究了芝麻油的成分芝麻酚对 UVB（280-320nm）照射的人血淋巴细胞的光保护作用。将淋巴细胞用不同浓度的芝麻酚（1、5 和 10μg/ml）预处理 30min，然后进行辐照，并检测脂质过氧化和抗氧化防御。UVB 照射的淋巴细胞表现出脂质过氧化水平升高和抗氧化防御紊乱。芝麻酚预处理导致脂质过氧化标志物硫代巴比妥酸反应物质（TBARS）显著减少。此外，抗氧化剂如还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）在芝麻酚预处理和 UVB 照射的淋巴细胞中呈剂量依赖性增加。最大剂量的芝麻酚（10μg/ml）使 UVB 诱导的脂质过氧化正常化，表明芝麻酚对辐照淋巴细胞具有光保护作用。
