Laboratory of Cellular Signaling, Faculty of Integrated Arts and Sciences, The University of Tokushima, Tokushima 770-8502, Japan.
Environ Toxicol Pharmacol. 2006 Sep;22(2):213-8. doi: 10.1016/j.etap.2006.03.007. Epub 2006 May 11.
Tri-n-butyltin (TBT), one of environmental pollutants, disturbs intracellular Ca(2+) homeostasis by increasing intracellular Ca(2+) concentration ([Ca(2+)]i). Effect of TBT on oscillatory change in [Ca(2+)]i (Ca(2+) oscillation) of rat thymocytes was examined using a laser microscope with fluo-3-AM in order to further elucidate the TBT toxicity related to intracellular Ca(2+). The Ca(2+) oscillation was completely attenuated by 300nM TBT. Since store-operated Ca(2+) channels are involved in the generation of Ca(2+) oscillation, the action of TBT on an increase in [Ca(2+)]i by Ca(2+) influx through store-operated Ca(2+) channels was examined. The increase in [Ca(2+)]i by the store-operated Ca(2+) influx was not affected by 3nM TBT. However, TBT at 10nM or more significantly reduced the increase in [Ca(2+)]i. It is likely that TBT attenuates the Ca(2+) oscillation by reducing the Ca(2+) influx through store-operated Ca(2+) channels.
三丁基锡（TBT）是一种环境污染物，通过增加细胞内钙离子浓度 ([Ca(2+)]i) 扰乱细胞内 Ca(2+) 稳态。为了进一步阐明与细胞内 Ca(2+) 相关的 TBT 毒性，使用带有 fluo-3-AM 的激光显微镜检查 TBT 对大鼠胸腺细胞中 [Ca(2+)]i（Ca(2+) 振荡）的振荡变化的影响。300nM TBT 完全阻断 Ca(2+) 振荡。由于储存操作的 Ca(2+) 通道参与 Ca(2+) 振荡的产生，因此检查 TBT 对通过储存操作的 Ca(2+) 通道的 Ca(2+) 内流引起的 [Ca(2+)]i 增加的作用。通过储存操作的 Ca(2+) 内流引起的 [Ca(2+)]i 的增加不受 3nM TBT 的影响。然而，TBT 在 10nM 或更高浓度时显著降低 [Ca(2+)]i 的增加。TBT 可能通过减少通过储存操作的 Ca(2+) 通道的 Ca(2+) 内流来减弱 Ca(2+) 振荡。
