Faculty of Environmental Engineering, College of Urban Science, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea.
Environ Toxicol Pharmacol. 2008 May;25(3):393-8. doi: 10.1016/j.etap.2007.12.006. Epub 2008 Jan 4.
In this study, a preliminary characterization of the multiplicity of Chironomus hemoglobin (Hb) was conducted on the larvae of Chironomus tentans Mg. (Diptera: Chironomidae) by the molecular mass and isoelectric point. In order to identify Chironomus Hb as potential biomarker of environmental contamination, alteration of individual Hb by exposure to environmental chemicals, namely, nonylphenol, benzo[a]pyrene, chloropyriphos and cadmium chloride, were evaluated. To validate the ecotoxicological relevance of Chironomus Hb as a potential biomarker, ecotoxicity test using growth rate as toxic endpoint was also conducted. This study revealed a striking heterogeneity in C. tentans Hb; 10 Hb isoforms were observed in the larvae of 4th instar C. tentans by their molecular mass and by their isoelectric point. Chemical-induced alteration of individual Hb expression suggests that C. tentans Hb can be a target molecule for chemical exposure. Overall results suggest that the expression pattern of Hb proteins may potentially contribute to the development of a biomarker for ecotoxicity monitoring in C. tentans.
本研究通过分子质量和等电点初步描述了四氯虫酰胺敏感品系（Mg）摇蚊幼虫的血红蛋白（Hb）的多样性。为了鉴定摇蚊 Hb 是否可作为环境污染的生物标志物，评估了环境化学物质（壬基酚、苯并[a]芘、毒死蜱和氯化镉）对个体 Hb 的改变。为了验证摇蚊 Hb 作为潜在生物标志物的生态毒理学相关性，还进行了以生长率为毒性终点的生态毒性测试。本研究表明，四氯虫酰胺敏感品系摇蚊 Hb 存在明显的异质性；通过分子质量和等电点观察到第 4 龄幼虫中存在 10 种 Hb 同工型。个体 Hb 表达的化学诱导改变表明，摇蚊 Hb 可能是化学暴露的靶分子。总体结果表明，Hb 蛋白的表达模式可能有助于开发用于监测摇蚊生态毒性的生物标志物。
