Department of Basic Pharmacology, Liaoning University of Traditional Chinese Medicine,110032 Shenyang, PR China; Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
Environ Toxicol Pharmacol. 2009 May;27(3):384-9. doi: 10.1016/j.etap.2009.01.001. Epub 2009 Jan 15.
The present study investigated the protective effects of ginsenoside Rb(1) (GRb(1)) against genotoxicity induced by cyclophosphamide (CP). Single cell gel electrophoresis, flow cytometry assay with annexin V-FITC/propidine iodide (PI) and acridine orange (AO)/ethidium bromide (EB) staining assay were employed to measure DNA damage and cell apoptosis, respectively. The activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GPx) and the malondialdehyde (MDA) content were also investigated by a number of colormetric methods. The results showed that the CP produced significant DNA damage and cell apoptosis in mouse bone marrow cells or peripheral blood leukocytes, markedly inhibited the activities of T-SOD and GPx, and markedly increased the MDA content. GRb(1) significantly inhibited DNA damages and cell apoptosis in mouse bone marrow cells or peripheral blood leukocytes induced by CP and antagonized the reduction of CP-induced T-SOD and GPx activities, and inhibited the increase in MDA content induced by CP. The anti-tumor study of GRb(1) showed that GRb(1) did not affect the anti-tumor activities of CP. In conclusion, GRb(1) had significant protective effects against DNA damage and apoptosis induced by CP.
本研究探讨了人参皂甙 Rb1（GRb1）对环磷酰胺（CP）诱导的遗传毒性的保护作用。采用单细胞凝胶电泳、流式细胞术结合 Annexin V-FITC/PI 和吖啶橙（AO）/溴化乙锭（EB）染色法分别检测 DNA 损伤和细胞凋亡。还采用多种比色法检测总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GPx）的活性以及丙二醛（MDA）的含量。结果表明，CP 可显著诱导小鼠骨髓细胞或外周血白细胞的 DNA 损伤和细胞凋亡，显著抑制 T-SOD 和 GPx 的活性，显著增加 MDA 的含量。GRb1 可显著抑制 CP 诱导的小鼠骨髓细胞或外周血白细胞的 DNA 损伤和细胞凋亡，并拮抗 CP 诱导的 T-SOD 和 GPx 活性降低，抑制 CP 诱导的 MDA 含量增加。GRb1 的抗肿瘤研究表明，GRb1 不影响 CP 的抗肿瘤活性。总之，GRb1 对 CP 诱导的 DNA 损伤和凋亡具有显著的保护作用。
