Zhejiang University, The Second Affiliated Hospital of Medical College, Institute of Oncology, Hangzhou 310002, Zhejiang, People's Republic of China.
Environ Toxicol Pharmacol. 2009 Sep;28(2):275-9. doi: 10.1016/j.etap.2009.05.001. Epub 2009 May 14.
In present study, human lymphocytes were exposed to cigarette smoke condensates (CSCs) at the doses of 25, 50, 75, 100 and 125 μg ml(-1) with and without S9, and the cyto-genotoxic effects were detected with CCK-8, cell apoptosis and micronucleus assays. DNA repair kinetics was observed with comet assay. Our results indicated that the cell viability decreased with CSCs doses, the percentages of apoptosis cell and the frequencies of micronuclei increased with CSCs doses, and DNA damage of human lymphocytes induced by CSCs could be basically repaired within 240 min. However, the cytotoxicity induced by CSCs +S9 was significantly lower than that induced by CSCs -S9 in CCK-8 and cell apoptosis assays, and the DNA repair speed in +S9 group was quicker than that in -S9 group. In conclusion, S9 may affect not only the cyto-genotoxicity of CSCs but also the repair process of DNA damage induced by CSCs in lymphocytes.
在本研究中，用人外周血淋巴细胞分别与不同浓度（25、50、75、100 和 125μg/ml）的香烟烟雾浓缩物（CSCs）共同孵育（有或无 S9），采用 CCK-8 法、细胞凋亡检测法和微核检测法观察 CSCs 对细胞的遗传毒性作用，采用彗星电泳检测法观察 DNA 损伤修复动力学。结果表明，CSCs 染毒剂量依赖性地降低细胞活力，诱导细胞凋亡和增加微核率，CSCs 诱导的人外周血淋巴细胞 DNA 损伤可在 240min 内基本修复；而 S9 混合物存在时，CSCs- S9 组细胞毒性作用明显高于 CSCs +S9 组，CSCs +S9 组细胞 DNA 修复速度明显快于 CSCs -S9 组。因此，S9 混合物不仅影响 CSCs 的遗传毒性作用，还影响 CSCs 诱导的 DNA 损伤的修复过程。
