Department of Pharmacology, University College of Medical Sciences, Dilshad Garden, Delhi 110095, India.
Environ Toxicol Pharmacol. 2009 Nov;28(3):403-8. doi: 10.1016/j.etap.2009.06.007. Epub 2009 Jul 7.
Propoxur, a carbamate pesticide has been shown to adversely affect memory and induce oxidative stress. The present study was designed to correlate the effect of propoxur, piracetam (a nootropic drug) and ascorbic acid (an antioxidant) on oxidative stress and cognitive function. Cognitive function was assessed using step-down latency (SDL) on a passive avoidance apparatus and transfer latency (TL) on elevated plus maze. Oxidative stress was assessed by examining brain malondialdehyde (MDA) and non-protein thiol (NP-SH) levels. A significant reduction in SDL and prolongation of TL was found for the propoxur-treated group at weeks 6 and 7 as compared with control (p<0.001). One week treatment by piracetam (400mg/kg/d, i.p.) or ascorbic acid (120mg/kg/d, i.p.) antagonized the effect of propoxur on SDL as well as TL. Both piracetam and ascorbic acid attenuated the propoxur-induced increase in brain MDA levels and decrease in brain NP-SH levels. Results of the present study show that ascorbic acid and piracetam have the potential to reverse cognitive dysfunction and oxidative stress induced by propoxur in the brain.
丙硫克百威是一种氨基甲酸酯类农药，已被证明会对记忆产生不良影响并诱导氧化应激。本研究旨在探讨丙硫克百威、吡拉西坦（一种促智药）和抗坏血酸（一种抗氧化剂）对氧化应激和认知功能的影响。使用被动回避装置上的步下潜伏期 (SDL) 和高架十字迷宫上的转移潜伏期 (TL) 评估认知功能。通过检查大脑丙二醛 (MDA) 和非蛋白巯基 (NP-SH) 水平来评估氧化应激。与对照组相比，丙硫克百威处理组在第 6 周和第 7 周时 SDL 显著降低，TL 延长（p<0.001）。吡拉西坦（400mg/kg/d，腹腔注射）或抗坏血酸（120mg/kg/d，腹腔注射）每周处理 1 次可拮抗丙硫克百威对 SDL 以及 TL 的作用。吡拉西坦和抗坏血酸均减轻了丙硫克百威引起的大脑 MDA 水平升高和 NP-SH 水平降低。本研究结果表明，抗坏血酸和吡拉西坦具有逆转丙硫克百威引起的大脑认知功能障碍和氧化应激的潜力。
