Dipartimento di Scienze del Farmaco, Università G. d'Annunzio, 66013 Chieti, Italy.
Chem Phys Lipids. 2011 Oct;164(7):680-7. doi: 10.1016/j.chemphyslip.2011.07.002. Epub 2011 Jul 18.
The cationic large unilamellar mixed liposomes from 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) and didodecyldimethylammonium bromide (DDAB) or dioctadecyldimethylammonium bromide (DODAB) were prepared. The influence of the addition of Triton X-100 (TX-100) or octaethylene glycol mono-n-dodecylether (C(12)E(8)) on the membrane integrity was investigated turbidimetrically. The stability of the liposomal systems was estimated by monitoring fluorimetrically at 25 °C the rate of spontaneous and surfactant-induced release of entrapped 5(6)-carboxyfluorescein (CF). In order to evaluate the interaction of the cationic DODAB guest with the host POPC membrane, the main phase transition temperatures (T(m)) were determined by electron paramagnetic resonance spectroscopy (EPR). All the results obtained show that the presence of DODAB and DDAB stabilizes the POPC liposomes. The extent of stabilization depends on the concentration and nature of the cationic guest.
从 1-棕榈酰基-2-油酰基-磷脂酰胆碱（POPC）和双十二烷基二甲基溴化铵（DDAB）或二油酰基二甲基溴化铵（DODAB）制备阳离子大单层混合脂质体。用浊度法研究了添加曲通 X-100（TX-100）或八乙二醇单十二醚（C（12）E（8））对膜完整性的影响。通过在 25°C 监测荧光法监测包封的 5（6）-羧基荧光素（CF）的自发和表面活性剂诱导释放速率，评估脂质体系统的稳定性。为了评估阳离子 DODAB 客体与宿主 POPC 膜的相互作用，通过电子顺磁共振波谱法（EPR）确定主要相变温度（T（m））。所有获得的结果均表明，DODAB 和 DDAB 的存在稳定了 POPC 脂质体。稳定程度取决于阳离子客体的浓度和性质。
