Oxford Expression Technologies Ltd., Oxford OX3 0BP, United Kingdom.
J Invertebr Pathol. 2011 Jul;107 Suppl:S49-58. doi: 10.1016/j.jip.2011.05.005.
The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is able to transduce a wide range of mammalian cells and shows preferential uptake in some, particularly liver and kidney cells. This suggests that the virus may be useful for delivery of protective genes for ameliorating the effects of ischaemia reperfusion injury (IRI) in solid organs during transplantation procedures. In this chapter we discuss the advantages of the baculovirus over other virus vectors for gene delivery in organ transplantation and describe some of the protective genes which may be used to ameliorate the effects of IRI. We then describe a method for concentrating baculovirus for use in an ex vivo transduction model. Data are also provided for the effects of virus transduction in vitro on the innate and adaptive immune response. We conclude with a discussion on the future considerations for using baculovirus for delivery and expression of protective genes in organ transplantation.
杆状病毒 Autographa californica 多角体核型多角体病毒 (AcMNPV) 能够转导广泛的哺乳动物细胞，并在某些细胞中表现出优先摄取，特别是肝脏和肾脏细胞。这表明该病毒可能可用于递送保护性基因，以减轻移植过程中实体器官缺血再灌注损伤 (IRI) 的影响。在本章中，我们讨论了杆状病毒在器官移植中的基因传递方面相对于其他病毒载体的优势，并描述了一些可能用于减轻 IRI 影响的保护性基因。然后，我们描述了一种浓缩杆状病毒用于离体转导模型的方法。还提供了病毒转导对体外固有和适应性免疫反应的影响的数据。最后，我们讨论了在器官移植中使用杆状病毒递送和表达保护性基因的未来考虑因素。
