Institute of Modern Physics, Chinese Academy of Sciences.
J Radiat Res. 2011;52(4):425-32. doi: 10.1269/jrr.10158.
Up to now, more than 1048 human miRNAs have been identified. However, the recognition of new human miRNAs is becoming more and more difficult. Based on the hypothesis that the expression of some miRNAs can be induced by ionizing radiation, total RNAs of HeLa cells were isolated 1 h after exposure to 2 Gy of X-rays, and total small RNAs were enriched and sequenced by PAGE and Solexa technology, respectively. As a result, 421 kinds of known miRNAs and 337 kinds of unknown sequences were identified, among which 10 novel miRNAs were characterized by bioinformatic approach and verified by qRT-PCR. Finally, putative targets of these miRNAs were predicted by TargetScan software and compared with known proteins down-regulated by radiation. It was confirmed that some of the targets of these novel miRNAs were radiation-related proteins. These results imply that these 10 novel miRNAs are radiation-related miRNAs. This study reveals a new way to find novel miRNAs.
到目前为止，已经鉴定出超过 1048 个人类 miRNA。然而，识别新的人类 miRNA 变得越来越困难。基于某些 miRNA 的表达可以被电离辐射诱导的假设，在 HeLa 细胞暴露于 2 Gy X 射线 1 小时后分离总 RNA，并分别通过 PAGE 和 Solexa 技术富集和测序总小分子 RNA。结果，鉴定出 421 种已知的 miRNA 和 337 种未知序列，其中通过生物信息学方法鉴定了 10 种新的 miRNA，并通过 qRT-PCR 进行了验证。最后，通过 TargetScan 软件预测这些 miRNA 的潜在靶标，并与辐射下调的已知蛋白进行比较。证实这些新 miRNA 的一些靶标是与辐射相关的蛋白。这些结果表明，这些新的 miRNA 是与辐射相关的 miRNA。本研究揭示了一种寻找新 miRNA 的新方法。
