Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes. - Suppr | 超能文献

Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes.

作者信息

Wang Chun-Wei, Kapoor Rakesh

机构信息

Department of Physics, University of Alabama at Birmingham, Birmingham, AL 35294.

出版信息

Proc SPIE Int Soc Opt Eng. 2010 Jan 23;7559(75590A). doi: 10.1117/12.843720.

DOI:10.1117/12.843720

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3140283/

Abstract

The document reports a novel method of measuring dissociation constant (k(D)) of antibody-antigen interaction using evanescent wave based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring the dissociation constant of human Interleukin-6 (IL-6) and anti-IL-6 interaction. Sandwich immunoassay was used to generate fluorescence signal proportional to antigen-antibody conjugate pairs. A portable CCD based spectrometer setup was used to record spectral profile of the fluorescence signal. The measured value of dissociation constant k(D) for IL-6 and capture anti-IL-6 (clone MQ2-13A5) antibodies at room temperature is588 ±19 pM .

摘要

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