单细胞电裂红细胞检测细胞骨架蛋白网络缺陷。
Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network.
机构信息
Department of Chemical Engineering, School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, Virginia 24061, USA.
出版信息
Lab Chip. 2011 Sep 21;11(18):3053-6. doi: 10.1039/c1lc20365g. Epub 2011 Jul 25.
Abstract
The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.
摘要
红细胞骨架蛋白网络显著影响红细胞的物理和生物学特性。在这里，我们表明，在电场暴露下红细胞裂解的动力学与细胞骨架网络缺陷密切相关。从单细胞电裂解数据编制的直方图显示了特定细胞骨架蛋白缺陷的红细胞群体的特征，揭示了细胞亚群的存在。
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