Department of Safety, Health and Environmental Engineering, Hungkuang University, Taichung, Taiwan.
Arch Toxicol. 2012 Jan;86(1):45-53. doi: 10.1007/s00204-011-0727-9. Epub 2011 Jul 22.
Urinary 3-hydroxy-benzo[a]pyrene (3-OHBaP) is considered as an exposure marker for assessing carcinogenic risks as it is a metabolite of benzo[a]pyrene (BaP). A controlled human study was conducted to examine the kinetics of urinary 3-OHBaP after consuming charcoal-barbecued meat. Two feeding experiments were performed, with meat doses of 15 and 30 g/kg (experiments 1 and 2, respectively). All voided urine over 7 days was collected and analyzed. The background urinary 3-OHBaP concentration was 0.002-0.085 ng/g creatinine, with large inter-individual (80-100%) and intra-individual of (90-100%) variations. The background amount of 3-OHBaP excreted by the current subjects was 40-50 pg/day. The amounts of urinary 3-OHBaP excreted within 12 h post-exposure increased significantly (P < 0.05) from background only in experiment 1. The proportion of the administered BaP dose that was excreted as urinary 3-OHBaP within 12 h post-exposure was 0.006 and 0.0012% in experiments 1 and 2, respectively. The excretion ratio declined as the dose increased. Urinary 3-OHBaP can be used to assess dietary exposure to BaP, but it may be not suitable for low-dose scenarios because of the low urinary excretion proportion and high variability of the background.
尿液 3-羟基苯并[a]芘（3-OHBaP）被认为是评估致癌风险的暴露标志物，因为它是苯并[a]芘（BaP）的代谢物。进行了一项对照人体研究，以研究摄入木炭烧烤肉后尿液 3-OHBaP 的动力学。进行了两项喂养实验，肉剂量分别为 15 和 30 g/kg（实验 1 和 2）。收集并分析了 7 天内所有的尿液。背景尿液 3-OHBaP 浓度为 0.002-0.085ng/g 肌酐，个体间（80-100%）和个体内（90-100%）变化较大。当前受试者排出的 3-OHBaP 背景量为 40-50pg/天。暴露后 12 小时内排出的尿液 3-OHBaP 量在实验 1 中仅从背景水平显著增加（P<0.05）。暴露后 12 小时内，作为尿液 3-OHBaP 排泄的给予 BaP 剂量的比例分别为实验 1 和 2 中的 0.006%和 0.0012%。随着剂量的增加，排泄比例下降。尿液 3-OHBaP 可用于评估饮食中 BaP 的暴露，但由于背景的低排泄比例和高变异性，它可能不适合低剂量情况。
