Production and Research Centre of Immunobiological Products, Parana State Department of Health, Paraná, Brazil.
Appl Microbiol Biotechnol. 2012 Jan;93(1):151-8. doi: 10.1007/s00253-011-3491-0. Epub 2011 Jul 23.
The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. The performance of three different inexpensive supports (vermiculite, sand, and sugarcane bagasse) was assessed by determining Bacillus atrophaeus sporulation during solid-state fermentation and by assessing the direct use of the fermentation products in the subsequent steps of the process. All three supports allowed spore production of between 10(7) and 10(9) CFU g(-1). Sand proved to be the best inert support enabling the direct use of the fermented product due to its easy homogenization, filling properties, and compatibility with recovery medium. Bacterial adhesion to the sand surface was supported by biofilm formation. The resistance to sterilization of the dried fermentation product was evaluated. For dry-heat resistance (160°C), the D value was 6.6 min, and for ethylene oxide resistance (650 mg/L), the D value was 6.5 min. The cost reduction of this process was at least 48%. No previous studies have been published on the application of sand as a support in solid-state fermentation for the production of biological indicators.
已经描述了涉及细菌孢子形成和多步下游工艺的生物指示剂的生产。本工作的目的是在生物指示剂中使用发酵材料作为最终产品，从而减少加工步骤和成本。通过确定固体发酵过程中萎缩芽孢杆菌的孢子形成，并评估发酵产物在后续步骤中的直接使用，评估了三种不同廉价载体（蛭石、砂和甘蔗渣）的性能。所有三种载体都允许产生 10(7) 到 10(9) CFU g(-1) 的孢子。由于砂具有易于均匀化、填充性能以及与回收介质的兼容性，因此被证明是最好的惰性载体，能够直接使用发酵产品。细菌对砂表面的粘附通过生物膜形成得到支持。评估了干燥发酵产物的耐灭菌性。对于干热抗性（160°C），D 值为 6.6 分钟，对于环氧乙烷抗性（650 mg/L），D 值为 6.5 分钟。该过程的成本降低至少为 48%。以前没有发表过关于砂作为固体发酵生产生物指示剂的载体的应用的研究。
