Zhirnov O P, Klenk H D
Vopr Virusol. 2011 May-Jun;56(3):24-8.
Infectivity of pandemic influenza virus A(H1N1) infectivity is shown to be activated through proteolytic cleavage of hemagglutinin HA0 --> HA1 + HA2 during virus propagation in the human intestinal cell line Caco-2 and chicken embryonated eggs. Injection of aprotinin, a natural serine protease inhibitor, into the liquid culture or allantoic cavity of chicken embryos inhibited the proteolysis of the viral HA0 and suppressed the proteolytic activation of the synthesized virus and its multicycle replication. These data allow aprotinin to be recommended as an antiviral drug for the treatment of swine influenza in humans.
甲型H1N1大流行性流感病毒的感染性在病毒于人类肠道细胞系Caco-2和鸡胚中繁殖期间，通过血凝素HA0裂解为HA1 + HA2的蛋白水解作用而被激活。向鸡胚的液体培养物或尿囊腔中注射天然丝氨酸蛋白酶抑制剂抑肽酶，可抑制病毒HA0的蛋白水解，并抑制合成病毒的蛋白水解激活及其多轮复制。这些数据表明抑肽酶可作为治疗人类猪流感的抗病毒药物。
